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mKikGR, a monomeric photoswitchable fluorescent protein

Journal Article


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Abstract


  • The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.

Authors


  •   Habuchi, Satoshi (external author)
  •   Tsutsui, Hidekazu (external author)
  •   Kochaniak, Anna B. (external author)
  •   Miyawaki, Atsushi (external author)
  •   van Oijen, Antoine M.

Publication Date


  • 2008

Citation


  • Habuchi, S., Tsutsui, H., Kochaniak, A. B., Miyawaki, A. & van Oijen, A. M. (2008). mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One, 3 (12), e3944-1-e3944-9.

Scopus Eid


  • 2-s2.0-58049208311

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3223&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2205

Has Global Citation Frequency


Start Page


  • e3944-1

End Page


  • e3944-9

Volume


  • 3

Issue


  • 12

Place Of Publication


  • United States

Abstract


  • The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.

Authors


  •   Habuchi, Satoshi (external author)
  •   Tsutsui, Hidekazu (external author)
  •   Kochaniak, Anna B. (external author)
  •   Miyawaki, Atsushi (external author)
  •   van Oijen, Antoine M.

Publication Date


  • 2008

Citation


  • Habuchi, S., Tsutsui, H., Kochaniak, A. B., Miyawaki, A. & van Oijen, A. M. (2008). mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One, 3 (12), e3944-1-e3944-9.

Scopus Eid


  • 2-s2.0-58049208311

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3223&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2205

Has Global Citation Frequency


Start Page


  • e3944-1

End Page


  • e3944-9

Volume


  • 3

Issue


  • 12

Place Of Publication


  • United States