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CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule

Journal Article


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Abstract


  • Spatial regulation of microtubule (MT) dynamics contributes to cell polarity and cell division. MT rescue, in which a MT stops shrinking and reinitiates growth, is the least understood aspect of MT dynamics. Cytoplasmic Linker Associated Proteins (CLASPs) are a conserved class of MT-associated proteins that contribute to MT stabilization and rescue in vivo. We show here that the Schizosaccharomyces pombe CLASP, Cls1p, is a homodimer that binds an αβ-tubulin heterodimer through conserved TOG-like domains. In vitro, CLASP increases MT rescue frequency, decreases MT catastrophe frequency, and moderately decreases MT disassembly rate. CLASP binds stably to the MT lattice, recruits tubulin, and locally promotes rescues. Mutations in the CLASP TOG domains demonstrate that tubulin binding is critical for its rescue activity. We propose a mechanism for rescue in which CLASP-tubulin dimer complexes bind along the MT lattice and reverse MT depolymerization with their bound tubulin dimer.

Authors


  •   Al-Bassam, Jawdat (external author)
  •   Kim, Hwajin (external author)
  •   Brouhard, Gary (external author)
  •   van Oijen, Antoine M.
  •   Harrison, Stephen C. (external author)
  •   Chang, Fred (external author)

Publication Date


  • 2010

Citation


  • Al-Bassam, J., Kim, H., Brouhard, G., van Oijen, A. M., Harrison, S. C. & Chang, F. (2010). CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule. Developmental Cell, 19 (2), 245-258.

Scopus Eid


  • 2-s2.0-77955588492

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3213&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2195

Has Global Citation Frequency


Number Of Pages


  • 13

Start Page


  • 245

End Page


  • 258

Volume


  • 19

Issue


  • 2

Place Of Publication


  • United States

Abstract


  • Spatial regulation of microtubule (MT) dynamics contributes to cell polarity and cell division. MT rescue, in which a MT stops shrinking and reinitiates growth, is the least understood aspect of MT dynamics. Cytoplasmic Linker Associated Proteins (CLASPs) are a conserved class of MT-associated proteins that contribute to MT stabilization and rescue in vivo. We show here that the Schizosaccharomyces pombe CLASP, Cls1p, is a homodimer that binds an αβ-tubulin heterodimer through conserved TOG-like domains. In vitro, CLASP increases MT rescue frequency, decreases MT catastrophe frequency, and moderately decreases MT disassembly rate. CLASP binds stably to the MT lattice, recruits tubulin, and locally promotes rescues. Mutations in the CLASP TOG domains demonstrate that tubulin binding is critical for its rescue activity. We propose a mechanism for rescue in which CLASP-tubulin dimer complexes bind along the MT lattice and reverse MT depolymerization with their bound tubulin dimer.

Authors


  •   Al-Bassam, Jawdat (external author)
  •   Kim, Hwajin (external author)
  •   Brouhard, Gary (external author)
  •   van Oijen, Antoine M.
  •   Harrison, Stephen C. (external author)
  •   Chang, Fred (external author)

Publication Date


  • 2010

Citation


  • Al-Bassam, J., Kim, H., Brouhard, G., van Oijen, A. M., Harrison, S. C. & Chang, F. (2010). CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule. Developmental Cell, 19 (2), 245-258.

Scopus Eid


  • 2-s2.0-77955588492

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3213&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2195

Has Global Citation Frequency


Number Of Pages


  • 13

Start Page


  • 245

End Page


  • 258

Volume


  • 19

Issue


  • 2

Place Of Publication


  • United States