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Uncoupling of sister replisomes during eukaryotic DNA replication

Journal Article


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Abstract


  • The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this “double replisome” model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.

Authors


  •   Yardimci, Hasan (external author)
  •   Loveland, Anna B. (external author)
  •   Habuchi, Satoshi (external author)
  •   van Oijen, Antoine M.
  •   Walter, Johannes C. (external author)

Publication Date


  • 2010

Citation


  • Yardimci, H., Loveland, A. B., Habuchi, S., van Oijen, A. M. & Walter, J. C. (2010). Uncoupling of sister replisomes during eukaryotic DNA replication. Molecular Cell, 40 (5), 834-840.

Scopus Eid


  • 2-s2.0-78649969382

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3212&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2194

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 834

End Page


  • 840

Volume


  • 40

Issue


  • 5

Place Of Publication


  • United States

Abstract


  • The duplication of eukaryotic genomes involves the replication of DNA from multiple origins of replication. In S phase, two sister replisomes assemble at each active origin, and they replicate DNA in opposite directions. Little is known about the functional relationship between sister replisomes. Some data imply that they travel away from one another and thus function independently. Alternatively, sister replisomes may form a stationary, functional unit that draws parental DNA toward itself. If this “double replisome” model is correct, a constrained DNA molecule should not undergo replication. To test this prediction, lambda DNA was stretched and immobilized at both ends within a microfluidic flow cell. Upon exposure to Xenopus egg extracts, this DNA underwent extensive replication by a single pair of diverging replisomes. The data show that there is no obligatory coupling between sister replisomes and, together with other studies, imply that genome duplication involves autonomously functioning replisomes.

Authors


  •   Yardimci, Hasan (external author)
  •   Loveland, Anna B. (external author)
  •   Habuchi, Satoshi (external author)
  •   van Oijen, Antoine M.
  •   Walter, Johannes C. (external author)

Publication Date


  • 2010

Citation


  • Yardimci, H., Loveland, A. B., Habuchi, S., van Oijen, A. M. & Walter, J. C. (2010). Uncoupling of sister replisomes during eukaryotic DNA replication. Molecular Cell, 40 (5), 834-840.

Scopus Eid


  • 2-s2.0-78649969382

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3212&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2194

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 834

End Page


  • 840

Volume


  • 40

Issue


  • 5

Place Of Publication


  • United States