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DNA is a co-factor for its own replication in Xenopus egg extracts

Journal Article


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Abstract


  • Soluble Xenopus egg extracts efficiently replicate added plasmids using a physiological mechanism, and thus represent a powerful system to understand vertebrate DNA replication. Surprisingly, DNA replication in this system is highly sensitive to plasmid concentration, being undetectable below ∼10 pM and highly efficient above ∼75 pM. DNA replication at the high plasmid concentration does not require plasmid–plasmid contacts, since replication is not inhibited when plasmids are immobilized in agarose prior to addition of egg extract. The absence of replication at low plasmid concentration is due to a defect in the assembly of pre-replication complexes (pre-RCs). pre-RC assembly requires contact-independent communication between plasmids. Our results show that in Xenopus egg extracts, aggregation of multiple replication forks is not required for efficient replication of plasmid DNA, and they suggest that DNA functions as a co-factor for its own duplication.

Authors


  •   Lebofsky, Ronald (external author)
  •   van Oijen, Antoine M.
  •   Walter, Johannes C. (external author)

Publication Date


  • 2011

Citation


  • Lebofsky, R., van Oijen, A. M. & Walter, J. C. (2011). DNA is a co-factor for its own replication in Xenopus egg extracts. Nucleic Acids Research, 39 (2), 545-555.

Scopus Eid


  • 2-s2.0-79551501163

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3210&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2192

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 545

End Page


  • 555

Volume


  • 39

Issue


  • 2

Place Of Publication


  • United Kingdom

Abstract


  • Soluble Xenopus egg extracts efficiently replicate added plasmids using a physiological mechanism, and thus represent a powerful system to understand vertebrate DNA replication. Surprisingly, DNA replication in this system is highly sensitive to plasmid concentration, being undetectable below ∼10 pM and highly efficient above ∼75 pM. DNA replication at the high plasmid concentration does not require plasmid–plasmid contacts, since replication is not inhibited when plasmids are immobilized in agarose prior to addition of egg extract. The absence of replication at low plasmid concentration is due to a defect in the assembly of pre-replication complexes (pre-RCs). pre-RC assembly requires contact-independent communication between plasmids. Our results show that in Xenopus egg extracts, aggregation of multiple replication forks is not required for efficient replication of plasmid DNA, and they suggest that DNA functions as a co-factor for its own duplication.

Authors


  •   Lebofsky, Ronald (external author)
  •   van Oijen, Antoine M.
  •   Walter, Johannes C. (external author)

Publication Date


  • 2011

Citation


  • Lebofsky, R., van Oijen, A. M. & Walter, J. C. (2011). DNA is a co-factor for its own replication in Xenopus egg extracts. Nucleic Acids Research, 39 (2), 545-555.

Scopus Eid


  • 2-s2.0-79551501163

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3210&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2192

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 545

End Page


  • 555

Volume


  • 39

Issue


  • 2

Place Of Publication


  • United Kingdom