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A single molecule DNA flow stretching microscope for undergraduates

Journal Article


Abstract


  • The design of a simple, safe, and inexpensive single molecule flow stretching instrument is presented. The instrument uses a low cost upright microscope coupled to a webcam for imaging single DNA molecules that are tethered in an easy to construct microfluidic flow cell. The system requires no special vibration isolation and is capable of measuring DNA replication at the single molecule level. We discuss two laboratory experiments suitable for advanced undergraduates using our microscope.

Authors


  •   Williams, Kelly (external author)
  •   Grafe, Brendan (external author)
  •   Burke, Kathryn M. (external author)
  •   Tanner, Nathan A. (external author)
  •   van Oijen, Antoine M.
  •   Loparo, Joseph J. (external author)
  •   Price, Allen C. (external author)

Publication Date


  • 2011

Citation


  • Williams, K., Grafe, B., Burke, K. M., Tanner, N. A., van Oijen, A. M., Loparo, J. J. & Price, A. C. (2011). A single molecule DNA flow stretching microscope for undergraduates. American Journal of Physics, 79 (11), 1112-1120.

Scopus Eid


  • 2-s2.0-80054761736

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2173

Number Of Pages


  • 8

Start Page


  • 1112

End Page


  • 1120

Volume


  • 79

Issue


  • 11

Abstract


  • The design of a simple, safe, and inexpensive single molecule flow stretching instrument is presented. The instrument uses a low cost upright microscope coupled to a webcam for imaging single DNA molecules that are tethered in an easy to construct microfluidic flow cell. The system requires no special vibration isolation and is capable of measuring DNA replication at the single molecule level. We discuss two laboratory experiments suitable for advanced undergraduates using our microscope.

Authors


  •   Williams, Kelly (external author)
  •   Grafe, Brendan (external author)
  •   Burke, Kathryn M. (external author)
  •   Tanner, Nathan A. (external author)
  •   van Oijen, Antoine M.
  •   Loparo, Joseph J. (external author)
  •   Price, Allen C. (external author)

Publication Date


  • 2011

Citation


  • Williams, K., Grafe, B., Burke, K. M., Tanner, N. A., van Oijen, A. M., Loparo, J. J. & Price, A. C. (2011). A single molecule DNA flow stretching microscope for undergraduates. American Journal of Physics, 79 (11), 1112-1120.

Scopus Eid


  • 2-s2.0-80054761736

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2173

Number Of Pages


  • 8

Start Page


  • 1112

End Page


  • 1120

Volume


  • 79

Issue


  • 11