This paper investigates the Quality of Experience (QoE) of multisensory media by analysing biosignals collected by electroencephalography (EEG) and eye gaze sensors and comparing with subjective ratings. Also investigated is the impact on QoE of various levels of synchronicity between the sensory effect and target video scene. Results confirm findings from previous research that show sensory effects added to videos increases the QoE rating. While there was no statistical difference observed for the QoE ratings for different levels of sensory effect synchronicity, an analysis of raw EEG data showed 25% more activity in the temporal lobe during asynchronous effects and 20–25% more activity in the occipital lobe during synchronous effects. The eye gaze data showed more deviation for a video with synchronous effects and the EEG showed correlating occipital lobe activity for this instance. These differences in physiological responses indicate sensory effect synchronicity may affect QoE despite subjective ratings appearing similar.