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High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device

Journal Article


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Abstract


  • In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 μl min−1 with a purity of [similar]99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min−1 and a massive throughput of 7 × 108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.

UOW Authors


  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Li, Weihua
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)

Publication Date


  • 2014

Citation


  • Zhang, J., Yan, S., Li, W., Alici, G. & Nguyen, N. (2014). High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device. RSC Advances: an international journal to further the chemical sciences, 4 (63), 33149-33159.

Scopus Eid


  • 2-s2.0-84905728346

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3900&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/2891

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 33149

End Page


  • 33159

Volume


  • 4

Issue


  • 63

Place Of Publication


  • United Kingdom

Abstract


  • In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 μl min−1 with a purity of [similar]99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min−1 and a massive throughput of 7 × 108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.

UOW Authors


  •   Zhang, Jun (external author)
  •   Yan, Sheng (external author)
  •   Li, Weihua
  •   Alici, Gursel
  •   Nguyen, Nam-Trung (external author)

Publication Date


  • 2014

Citation


  • Zhang, J., Yan, S., Li, W., Alici, G. & Nguyen, N. (2014). High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device. RSC Advances: an international journal to further the chemical sciences, 4 (63), 33149-33159.

Scopus Eid


  • 2-s2.0-84905728346

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3900&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/2891

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 33149

End Page


  • 33159

Volume


  • 4

Issue


  • 63

Place Of Publication


  • United Kingdom