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Biobanks for pluripotent stem cells

Chapter


Abstract


  • To date, over 700 hESC lines have been used in over 1450 published studies (International Stem Cell Registry (ISCR)), signifying remarkable progress in a rapidly growing field. However, the findings in a number of these reports have been controversial, with some difficult to reproduce. This situation may have resulted from many factors, including varying competencies for cell line handling, differing culture conditions and usage of cells that have undergone deleterious changes. This chapter provides an overview of the fundamental principles of hPSC banking. A detailed section on current practices by stem cell providers and a discussion about the future of banking are also presented.

Authors


  •   Luong, Mai X. (external author)
  •   Smith, Kelly P. (external author)
  •   Crook, Jeremy M.
  •   Stacey, Glyn N. (external author)

Publication Date


  • 2012

Citation


  • Luong, M. X., Smith, K. P., Crook, J. M. & Stacey, G. (2012). Biobanks for pluripotent stem cells. In J. F. Loring & S. E. Peterson (Eds.), Human Stem Cell Manual: A Laboratory Guide (pp. 105-125). United Kingdom: Elsevier Inc.

Scopus Eid


  • 2-s2.0-84883904163

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1180

Book Title


  • Human Stem Cell Manual: A Laboratory Guide

Has Global Citation Frequency


Start Page


  • 105

End Page


  • 125

Place Of Publication


  • United Kingdom

Abstract


  • To date, over 700 hESC lines have been used in over 1450 published studies (International Stem Cell Registry (ISCR)), signifying remarkable progress in a rapidly growing field. However, the findings in a number of these reports have been controversial, with some difficult to reproduce. This situation may have resulted from many factors, including varying competencies for cell line handling, differing culture conditions and usage of cells that have undergone deleterious changes. This chapter provides an overview of the fundamental principles of hPSC banking. A detailed section on current practices by stem cell providers and a discussion about the future of banking are also presented.

Authors


  •   Luong, Mai X. (external author)
  •   Smith, Kelly P. (external author)
  •   Crook, Jeremy M.
  •   Stacey, Glyn N. (external author)

Publication Date


  • 2012

Citation


  • Luong, M. X., Smith, K. P., Crook, J. M. & Stacey, G. (2012). Biobanks for pluripotent stem cells. In J. F. Loring & S. E. Peterson (Eds.), Human Stem Cell Manual: A Laboratory Guide (pp. 105-125). United Kingdom: Elsevier Inc.

Scopus Eid


  • 2-s2.0-84883904163

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/1180

Book Title


  • Human Stem Cell Manual: A Laboratory Guide

Has Global Citation Frequency


Start Page


  • 105

End Page


  • 125

Place Of Publication


  • United Kingdom