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A female-gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite

Journal Article


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Abstract


  • ATP-binding cassette (ABC) transporters serve a variety of physiological functions as well as play key roles in drug resistance. The genome of the human malaria parasite, Plasmodium falciparum, encodes multiple members of this family, one of which, gABCG2, is transcribed predominantly in the gametocyte stage. Here we use gene deletion and tagging to investigate the expression, localization and function of gABCG2. The protein is found in a single dot-like lipid-rich structure within female, but not male, gametocytes. gABCG2-knockout cell lines produce more gametocytes of both sexes. By contrast, cholesteryl esters, diacylglycerols and triacylglycerols are significantly reduced in gABCG2-knockout gametocyte stages. We propose a role for gABCG2 in the regulation of gametocyte numbers and in the accumulation of neutral lipids, which are likely important for parasite development in the insect stages of the parasite life cycle.

Authors


  •   Tran, Phuong T. (external author)
  •   Brown, Simon H. J.
  •   Mitchell, Todd
  •   Matuschewski, Kai (external author)
  •   Mcmillan, Paul J. (external author)
  •   Kirk, Kiaran (external author)
  •   Dixon, Matthew W. A. (external author)
  •   Maier, Alexander (external author)

Publication Date


  • 2014

Citation


  • Tran, P. N., Brown, S. H. J., Mitchell, T. W., Matuschewski, K., Mcmillan, P. J., Kirk, K., Dixon, M. W. A. & Maier, A. G. (2014). A female-gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite. Nature Communications, 5 1-13.

Scopus Eid


  • 2-s2.0-84923334618

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1496&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/479

Number Of Pages


  • 12

Start Page


  • 1

End Page


  • 13

Volume


  • 5

Abstract


  • ATP-binding cassette (ABC) transporters serve a variety of physiological functions as well as play key roles in drug resistance. The genome of the human malaria parasite, Plasmodium falciparum, encodes multiple members of this family, one of which, gABCG2, is transcribed predominantly in the gametocyte stage. Here we use gene deletion and tagging to investigate the expression, localization and function of gABCG2. The protein is found in a single dot-like lipid-rich structure within female, but not male, gametocytes. gABCG2-knockout cell lines produce more gametocytes of both sexes. By contrast, cholesteryl esters, diacylglycerols and triacylglycerols are significantly reduced in gABCG2-knockout gametocyte stages. We propose a role for gABCG2 in the regulation of gametocyte numbers and in the accumulation of neutral lipids, which are likely important for parasite development in the insect stages of the parasite life cycle.

Authors


  •   Tran, Phuong T. (external author)
  •   Brown, Simon H. J.
  •   Mitchell, Todd
  •   Matuschewski, Kai (external author)
  •   Mcmillan, Paul J. (external author)
  •   Kirk, Kiaran (external author)
  •   Dixon, Matthew W. A. (external author)
  •   Maier, Alexander (external author)

Publication Date


  • 2014

Citation


  • Tran, P. N., Brown, S. H. J., Mitchell, T. W., Matuschewski, K., Mcmillan, P. J., Kirk, K., Dixon, M. W. A. & Maier, A. G. (2014). A female-gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite. Nature Communications, 5 1-13.

Scopus Eid


  • 2-s2.0-84923334618

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1496&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/479

Number Of Pages


  • 12

Start Page


  • 1

End Page


  • 13

Volume


  • 5