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Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry

Journal Article


Abstract


  • The lipidome of the human lens is unique in that cholesterol and dihydrosphingomyelin are the dominant classes. Moreover, the lens lipidome is not static with dramatic changes in several sphingolipid classes associated with both aging and cataract. Accordingly, there is a clear need to expand knowledge of the molecular species that constitute the human lens sphingolipidome. In this study, human lens lipids have been extracted and separated by thin-layer chromatography (TLC). Direct analysis of the TLC plates by desorption electrospray ionisation–mass spectrometry (DESI–MS) allowed the detection over 30 species from 11 classes of sphingolipids. Significantly, novel classes of lens lipids including sulfatides, dihydrosulfatides, lactosylceramide sulfates and dihydrolactosylceramide sulfates were identified.

Publication Date


  • 2014

Citation


  • Seng, J., Ellis, S. R., Hughes, J. R., Maccarone, A. T., Truscott, R. J., Blanksby, S. J. & Mitchell, T. W. (2014). Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry. BBA: Molecular and Cell Biology of Lipids, 1841 (9), 1285-1291.

Scopus Eid


  • 2-s2.0-84903551707

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2303

Has Global Citation Frequency


Number Of Pages


  • 6
  • 6

Start Page


  • 1285

End Page


  • 1291

Volume


  • 1841

Issue


  • 9

Place Of Publication


  • Netherlands

Abstract


  • The lipidome of the human lens is unique in that cholesterol and dihydrosphingomyelin are the dominant classes. Moreover, the lens lipidome is not static with dramatic changes in several sphingolipid classes associated with both aging and cataract. Accordingly, there is a clear need to expand knowledge of the molecular species that constitute the human lens sphingolipidome. In this study, human lens lipids have been extracted and separated by thin-layer chromatography (TLC). Direct analysis of the TLC plates by desorption electrospray ionisation–mass spectrometry (DESI–MS) allowed the detection over 30 species from 11 classes of sphingolipids. Significantly, novel classes of lens lipids including sulfatides, dihydrosulfatides, lactosylceramide sulfates and dihydrolactosylceramide sulfates were identified.

Publication Date


  • 2014

Citation


  • Seng, J., Ellis, S. R., Hughes, J. R., Maccarone, A. T., Truscott, R. J., Blanksby, S. J. & Mitchell, T. W. (2014). Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry. BBA: Molecular and Cell Biology of Lipids, 1841 (9), 1285-1291.

Scopus Eid


  • 2-s2.0-84903551707

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/2303

Has Global Citation Frequency


Number Of Pages


  • 6
  • 6

Start Page


  • 1285

End Page


  • 1291

Volume


  • 1841

Issue


  • 9

Place Of Publication


  • Netherlands