In order to analyze the mechanical behaviour of ultra-low carbon steel in warm rolling, the flow stress and pyroplastric behaviour were investigated on the Gleeble-3500 with the deformation temperatures in the warm range. The strain rates of 0.1, 1 and 10s-1 were applied to the temperatures above. A series of stress-strain curves were obtained after the compression Gleeble tests with different parameters. The result indicates the flow stress increases with the decreasing deformation temperatures during the compression. And it increases with the increasing strain rate as well. The constitutive equation, which includes the deformation activation energy Q and the Kelvin temperature T, was developed by using the concept that proposed by C. M. Sellars and W.J. M. Tegart. These equation results can be verified by the results of Gleeble compression test. It indicates the average error is reasonable and acceptable for predicting the flow stress and pyroplastic deformation behaviour of ultra-low carbon steel in warm temperature range. The achieved constitutive equation provides theoretical fundaments for designing the warm rolling process of ultra-low carbon steel. The experimental data also can be used as material property parameters for the establishment of finite element (FE) model. © (2014) Trans Tech Publications, Switzerland.