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Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration

Journal Article


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Abstract


  • The performance of two redox mediating compounds, namely 1-hydroxybenzotriazole (HBT) and syringaldehyde (SA), was compared in terms of enhancement of enzymatic degradation of a diverse set of 14 phenolic and 16 non-phenolic trace organic contaminants (TrOCs) and the toxicity of the treated media. Extracellular enzyme extract (predominantly containing laccase) from Trametes versicolor culture achieved efficient degradation (70-95%) of nine phenolic and one non-phenolic TrOCs. Mediator dosing extended the spectrum of efficiently degraded TrOCs to 13 phenolic and three non-phenolic compounds, with moderate improvements in removal of a few other non-phenolic compounds. TrOC removal efficiency improved significantly as the HBT dose was increased from 0.1 to 0.5 mM, while SA achieved similar removal over dosage range of 0.1-1 mM. A particular concern was the toxicity of the treated media (1200-2200 times that of the control) for all SA dosages applied. Overall, HBT at a concentration of 0.5 mM achieved the best removal without raising concern regarding toxicity of the treated media. The results are discussed in the light of the redox potential of the enzyme-mediator cocktail, the balance between the stability and reactivity of the radicals generated and their cytotoxic effects.

Authors


  •   Nguyen, Luong (external author)
  •   Hai, Faisal I.
  •   Kang, Jinguo (external author)
  •   Leusch, Frederic (external author)
  •   Roddick, Felicity (external author)
  •   Magram, Saleh Faraj (external author)
  •   Price, William E.
  •   Nghiem, Long D. (external author)

Publication Date


  • 2014

Citation


  • Nguyen, N. Luong., Hai, F. I., Kang, J., Leusch, F., Roddick, F., Magram, S. Faraj., Price, W. E. & Nghiem, L. D. (2014). Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration. Journal of the Taiwan Institute of Chemical Engineers, 45 (4), 1855-1862.

Scopus Eid


  • 2-s2.0-84903374032

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3504&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/2495

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1855

End Page


  • 1862

Volume


  • 45

Issue


  • 4

Place Of Publication


  • Netherlands

Abstract


  • The performance of two redox mediating compounds, namely 1-hydroxybenzotriazole (HBT) and syringaldehyde (SA), was compared in terms of enhancement of enzymatic degradation of a diverse set of 14 phenolic and 16 non-phenolic trace organic contaminants (TrOCs) and the toxicity of the treated media. Extracellular enzyme extract (predominantly containing laccase) from Trametes versicolor culture achieved efficient degradation (70-95%) of nine phenolic and one non-phenolic TrOCs. Mediator dosing extended the spectrum of efficiently degraded TrOCs to 13 phenolic and three non-phenolic compounds, with moderate improvements in removal of a few other non-phenolic compounds. TrOC removal efficiency improved significantly as the HBT dose was increased from 0.1 to 0.5 mM, while SA achieved similar removal over dosage range of 0.1-1 mM. A particular concern was the toxicity of the treated media (1200-2200 times that of the control) for all SA dosages applied. Overall, HBT at a concentration of 0.5 mM achieved the best removal without raising concern regarding toxicity of the treated media. The results are discussed in the light of the redox potential of the enzyme-mediator cocktail, the balance between the stability and reactivity of the radicals generated and their cytotoxic effects.

Authors


  •   Nguyen, Luong (external author)
  •   Hai, Faisal I.
  •   Kang, Jinguo (external author)
  •   Leusch, Frederic (external author)
  •   Roddick, Felicity (external author)
  •   Magram, Saleh Faraj (external author)
  •   Price, William E.
  •   Nghiem, Long D. (external author)

Publication Date


  • 2014

Citation


  • Nguyen, N. Luong., Hai, F. I., Kang, J., Leusch, F., Roddick, F., Magram, S. Faraj., Price, W. E. & Nghiem, L. D. (2014). Enhancement of trace organic contaminant degradation by crude enzyme extract from Trametes versicolor culture: Effect of mediator type and concentration. Journal of the Taiwan Institute of Chemical Engineers, 45 (4), 1855-1862.

Scopus Eid


  • 2-s2.0-84903374032

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=3504&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/2495

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1855

End Page


  • 1862

Volume


  • 45

Issue


  • 4

Place Of Publication


  • Netherlands