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P2X7 receptor activation induces CD62L shedding from human CD4+ and CD8+ T cells

Journal Article


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Abstract


  • The P2X7 receptor is expressed on T cells, however knowledge of its presence and function on human CD4+ and CD8+ subsets is limited. Immunolabeling with an anti-human P2X7 monoclonal antibody and flow cytometry demonstrated that P2X7 is present on the cell-surface of peripheral blood CD4+ and CD8+ T cells. Time-resolved flow cytometry demonstrated that extracellular ATP induced ethidium+ uptake into both T cell subsets. Flow cytometric measurements also demonstrated that ATP induced the rapid loss of CD62L (L-selectin) from CD4+ and CD8+ T cells. ATP-induced ethidium+ uptake and CD62L shedding were dramatically impaired in CD4+ and CD8+ T cells homozygous for the Glu496Ala loss-of-function single nucleotide polymorphism in the P2RX7 gene, demonstrating that both processes were a result of P2X7 activation. In summary, these results show that both human CD4+ and CD8+ T cells express P2X7 receptors, and that ATP activation of this receptor can lead to the rapid shedding of CD62L from these cells.

Publication Date


  • 2014

Citation


  • Sluyter, R. & Wiley, J. S. (2014). P2X7 receptor activation induces CD62L shedding from human CD4+ and CD8+ T cells. Inflammation and Cell Signaling, 1 (3), 44-49.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2828&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1810

Number Of Pages


  • 5

Start Page


  • 44

End Page


  • 49

Volume


  • 1

Issue


  • 3

Abstract


  • The P2X7 receptor is expressed on T cells, however knowledge of its presence and function on human CD4+ and CD8+ subsets is limited. Immunolabeling with an anti-human P2X7 monoclonal antibody and flow cytometry demonstrated that P2X7 is present on the cell-surface of peripheral blood CD4+ and CD8+ T cells. Time-resolved flow cytometry demonstrated that extracellular ATP induced ethidium+ uptake into both T cell subsets. Flow cytometric measurements also demonstrated that ATP induced the rapid loss of CD62L (L-selectin) from CD4+ and CD8+ T cells. ATP-induced ethidium+ uptake and CD62L shedding were dramatically impaired in CD4+ and CD8+ T cells homozygous for the Glu496Ala loss-of-function single nucleotide polymorphism in the P2RX7 gene, demonstrating that both processes were a result of P2X7 activation. In summary, these results show that both human CD4+ and CD8+ T cells express P2X7 receptors, and that ATP activation of this receptor can lead to the rapid shedding of CD62L from these cells.

Publication Date


  • 2014

Citation


  • Sluyter, R. & Wiley, J. S. (2014). P2X7 receptor activation induces CD62L shedding from human CD4+ and CD8+ T cells. Inflammation and Cell Signaling, 1 (3), 44-49.

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2828&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1810

Number Of Pages


  • 5

Start Page


  • 44

End Page


  • 49

Volume


  • 1

Issue


  • 3