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Extracellular adenosine 5'-triphosphate and lipopolysaccharide induce interleukin-1β release in canine blood

Journal Article


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Abstract


  • Binding of extracellular adenosine 5′-triphosphate (ATP) or lipopolysaccharide (LPS) to the damage-associated molecular pattern receptor P2X7 or the pathogen-associated molecular pattern receptor Toll-like receptor (TLR)4, respectively, can induce the release of the pleiotropic cytokine interleukin (IL)-1β in humans and mice. However, the release of IL-1β in dogs remains poorly defined. Using a canine IL-1β enzyme-linked immunosorbent assay, this study investigated whether ATP or LPS could induce IL-1β release in a canine blood-based assay. Short-term incubations (30 min) with ATP induced IL-1β release in LPS-primed canine blood, and this process could be near-completely impaired by the P2X7 antagonist, A438079. In contrast, ATP failed to induce IL-1β release from blood not primed with LPS. ATP-induced IL-1β release was observed with LPS-primed blood from eight different pedigrees or cross breeds. Long-term incubations (24 h) with LPS induced IL-1β release in canine blood in a concentration-dependent manner. This process was not altered by co-incubation with A438079. LPS-induced IL-1β release was observed with blood from 10 different pedigrees or cross breeds. These results demonstrate that both extracellular ATP and LPS can induce IL-1β release in dogs, and that ATP- but not LPS-induced IL-1β release in blood is dependent on P2X7 activation. These findings support the role of both P2X7 and TLR4 in IL-1β release in dogs.

Authors


  •   Spildrejorde, Mari (external author)
  •   Curtis, Stephen J. (external author)
  •   Curtis, Belinda L. (external author)
  •   Sluyter, Ronald

Publication Date


  • 2014

Citation


  • Spildrejorde, M., Curtis, S. J., Curtis, B. L. & Sluyter, R. (2014). Extracellular adenosine 5'-triphosphate and lipopolysaccharide induce interleukin-1β release in canine blood. Veterinary Immunology and Immunopathology, 157 (1-2), 105-110.

Scopus Eid


  • 2-s2.0-84890138631

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2345&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1327

Number Of Pages


  • 5

Start Page


  • 105

End Page


  • 110

Volume


  • 157

Issue


  • 1-2

Abstract


  • Binding of extracellular adenosine 5′-triphosphate (ATP) or lipopolysaccharide (LPS) to the damage-associated molecular pattern receptor P2X7 or the pathogen-associated molecular pattern receptor Toll-like receptor (TLR)4, respectively, can induce the release of the pleiotropic cytokine interleukin (IL)-1β in humans and mice. However, the release of IL-1β in dogs remains poorly defined. Using a canine IL-1β enzyme-linked immunosorbent assay, this study investigated whether ATP or LPS could induce IL-1β release in a canine blood-based assay. Short-term incubations (30 min) with ATP induced IL-1β release in LPS-primed canine blood, and this process could be near-completely impaired by the P2X7 antagonist, A438079. In contrast, ATP failed to induce IL-1β release from blood not primed with LPS. ATP-induced IL-1β release was observed with LPS-primed blood from eight different pedigrees or cross breeds. Long-term incubations (24 h) with LPS induced IL-1β release in canine blood in a concentration-dependent manner. This process was not altered by co-incubation with A438079. LPS-induced IL-1β release was observed with blood from 10 different pedigrees or cross breeds. These results demonstrate that both extracellular ATP and LPS can induce IL-1β release in dogs, and that ATP- but not LPS-induced IL-1β release in blood is dependent on P2X7 activation. These findings support the role of both P2X7 and TLR4 in IL-1β release in dogs.

Authors


  •   Spildrejorde, Mari (external author)
  •   Curtis, Stephen J. (external author)
  •   Curtis, Belinda L. (external author)
  •   Sluyter, Ronald

Publication Date


  • 2014

Citation


  • Spildrejorde, M., Curtis, S. J., Curtis, B. L. & Sluyter, R. (2014). Extracellular adenosine 5'-triphosphate and lipopolysaccharide induce interleukin-1β release in canine blood. Veterinary Immunology and Immunopathology, 157 (1-2), 105-110.

Scopus Eid


  • 2-s2.0-84890138631

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2345&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1327

Number Of Pages


  • 5

Start Page


  • 105

End Page


  • 110

Volume


  • 157

Issue


  • 1-2