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A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain

Journal Article


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Abstract


  • Ethnopharmacological relevance

    The aerial components of Meconopsis simplicifolia (D. Don) Walpers are indicated in Bhutanese traditional medicine for treating malaria, coughs and colds, and the infections of the liver, lung and blood. This study is to validate the ethnopharmacological uses of this plant and also identify potent antimalarial drug leads through bioassays of its crude extracts and phytochemical constituents.

    Materials and methods

    Meconopsis simplicifolia (D. Don) Walpers was collected from Bhutan and its crude MeOH extract was subjected to acid-base fractionation. Through repeated extractions, separations and spectroscopic analysis, the alkaloids obtained were identified and tested for their antimalarial and cytotoxicity activities.

    Results

    Phytochemical studies resulted in the isolation of one new protoberberine type alkaloid which we named as simplicifolianine and five known alkaloids: protopine, norsanguinarine, dihydrosanguinarine, 6-methoxydihydrosanguinarine and oxysanguinarine. Among the five of the alkaloids tested, simplicifolianine showed the most potent antiplasmodial activities against the Plasmodium falciparum strains, TM4/8.2 (chloroquine–antifolate sensitive strain) and K1CB1 (multidrug resistant strain) with IC50 values of 0.78 μg/mL and 1.29 μg/mL, respectively. The compounds tested did not show any significant cytotoxicity activities against human oral carcinoma KB cells and normal Vero cells of African kidney epithelial cells.

    Conclusions

    This study validated the traditional uses of the plant for the treatment of malaria and identified a new alkaloid, simplicifolianine as a potential antimalarial drug lead.

Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul A.
  •   Pyne, Stephen G.
  •   Lie, Wilford
  •   Willis, Anthony C. (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Lie, W., Willis, A. C., Rattanajak, R. & Kamchonwongpaisan, S. (2013). A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain. Journal of Ethnopharmacology, 150 (3), 953-959.

Scopus Eid


  • 2-s2.0-84889091616

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2286&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1268

Number Of Pages


  • 6

Start Page


  • 953

End Page


  • 959

Volume


  • 150

Issue


  • 3

Abstract


  • Ethnopharmacological relevance

    The aerial components of Meconopsis simplicifolia (D. Don) Walpers are indicated in Bhutanese traditional medicine for treating malaria, coughs and colds, and the infections of the liver, lung and blood. This study is to validate the ethnopharmacological uses of this plant and also identify potent antimalarial drug leads through bioassays of its crude extracts and phytochemical constituents.

    Materials and methods

    Meconopsis simplicifolia (D. Don) Walpers was collected from Bhutan and its crude MeOH extract was subjected to acid-base fractionation. Through repeated extractions, separations and spectroscopic analysis, the alkaloids obtained were identified and tested for their antimalarial and cytotoxicity activities.

    Results

    Phytochemical studies resulted in the isolation of one new protoberberine type alkaloid which we named as simplicifolianine and five known alkaloids: protopine, norsanguinarine, dihydrosanguinarine, 6-methoxydihydrosanguinarine and oxysanguinarine. Among the five of the alkaloids tested, simplicifolianine showed the most potent antiplasmodial activities against the Plasmodium falciparum strains, TM4/8.2 (chloroquine–antifolate sensitive strain) and K1CB1 (multidrug resistant strain) with IC50 values of 0.78 μg/mL and 1.29 μg/mL, respectively. The compounds tested did not show any significant cytotoxicity activities against human oral carcinoma KB cells and normal Vero cells of African kidney epithelial cells.

    Conclusions

    This study validated the traditional uses of the plant for the treatment of malaria and identified a new alkaloid, simplicifolianine as a potential antimalarial drug lead.

Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul A.
  •   Pyne, Stephen G.
  •   Lie, Wilford
  •   Willis, Anthony C. (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Lie, W., Willis, A. C., Rattanajak, R. & Kamchonwongpaisan, S. (2013). A new protoberberine alkaloid from Meconopsis simplicifolia (D. Don) Walpers with potent antimalarial activity against a multidrug resistant Plasmodium falciparum strain. Journal of Ethnopharmacology, 150 (3), 953-959.

Scopus Eid


  • 2-s2.0-84889091616

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2286&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1268

Number Of Pages


  • 6

Start Page


  • 953

End Page


  • 959

Volume


  • 150

Issue


  • 3