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High hydrostatic pressure effects investigated by neutron scattering on lipid multilamellar vesicles

Journal Article


Abstract


  • The effects of high hydrostatic pressure on the structure and dynamics of model membrane systems were investigated using neutron scattering. Diffraction experiments show shifts of the pre- and main-phase transitions of multilamellar vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) to higher temperatures with increased pressure which are close to results observed previously by other techniques, namely (10.4 ± 1.0) K kbar−1 and (20.0 ± 0.5) K kbar−1 for the two transitions. Backscattering spectroscopy reveals that the mean square displacements in the liquid phase are about 10% smaller at 300 bar and about 20% smaller at 600 bar compared to atmospheric pressure, whereas in the gel phase below the main phase transition the mean square displacements show a smaller difference in the dynamics of the three pressure values within the studied pressure range.

Authors


  •   Trapp, Marcus (external author)
  •   Marion, Jeremie (external author)
  •   Tehei, Moeava
  •   Demé, Bruno (external author)
  •   Gutberlet, Thomas (external author)
  •   Peters, Judith (external author)

Publication Date


  • 2013

Citation


  • Trapp, M., Marion, J., Tehei, M., Demé, B., Gutberlet, T. & Peters, J. (2013). High hydrostatic pressure effects investigated by neutron scattering on lipid multilamellar vesicles. Physical Chemistry Chemical Physics, 15 (48), 20951-20956.

Scopus Eid


  • 2-s2.0-84888213463

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1302

Number Of Pages


  • 5

Start Page


  • 20951

End Page


  • 20956

Volume


  • 15

Issue


  • 48

Abstract


  • The effects of high hydrostatic pressure on the structure and dynamics of model membrane systems were investigated using neutron scattering. Diffraction experiments show shifts of the pre- and main-phase transitions of multilamellar vesicles of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) to higher temperatures with increased pressure which are close to results observed previously by other techniques, namely (10.4 ± 1.0) K kbar−1 and (20.0 ± 0.5) K kbar−1 for the two transitions. Backscattering spectroscopy reveals that the mean square displacements in the liquid phase are about 10% smaller at 300 bar and about 20% smaller at 600 bar compared to atmospheric pressure, whereas in the gel phase below the main phase transition the mean square displacements show a smaller difference in the dynamics of the three pressure values within the studied pressure range.

Authors


  •   Trapp, Marcus (external author)
  •   Marion, Jeremie (external author)
  •   Tehei, Moeava
  •   Demé, Bruno (external author)
  •   Gutberlet, Thomas (external author)
  •   Peters, Judith (external author)

Publication Date


  • 2013

Citation


  • Trapp, M., Marion, J., Tehei, M., Demé, B., Gutberlet, T. & Peters, J. (2013). High hydrostatic pressure effects investigated by neutron scattering on lipid multilamellar vesicles. Physical Chemistry Chemical Physics, 15 (48), 20951-20956.

Scopus Eid


  • 2-s2.0-84888213463

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1302

Number Of Pages


  • 5

Start Page


  • 20951

End Page


  • 20956

Volume


  • 15

Issue


  • 48