Skip to main content
placeholder image

Glutathionylation potentiates benign superoxide dismutase 1 variants to the toxic forms associated with amyotrophic lateral sclerosis

Journal Article


Download full-text (Open Access)

Abstract


  • Dissociation of superoxide dismutase 1 dimers is enhanced by glutathionylation, although the dissociation constants reported to date are imprecise. We have quantified the discreet dissociation constants for wild-type superoxide dismutase 1 and six naturally occurring sequence variants, in their unmodified and glutathionylated forms, at the ratios expressed. Unmodified superoxide dismutase 1 variants that shared similar dissociation constants with SOD1WT had disproportionately increased dissociation constants when glutathionylated. This defines a key role for glutathionylation in superoxide dismutase 1 associated familial amyotrophic lateral sclerosis.

Publication Date


  • 2013

Citation


  • McAlary, L., Yerbury, J. J. & Aquilina, J. (2013). Glutathionylation potentiates benign superoxide dismutase 1 variants to the toxic forms associated with amyotrophic lateral sclerosis. Scientific Reports, 3 3275-1-3275-6.

Scopus Eid


  • 2-s2.0-84888218077

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2249&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1231

Start Page


  • 3275-1

End Page


  • 3275-6

Volume


  • 3

Abstract


  • Dissociation of superoxide dismutase 1 dimers is enhanced by glutathionylation, although the dissociation constants reported to date are imprecise. We have quantified the discreet dissociation constants for wild-type superoxide dismutase 1 and six naturally occurring sequence variants, in their unmodified and glutathionylated forms, at the ratios expressed. Unmodified superoxide dismutase 1 variants that shared similar dissociation constants with SOD1WT had disproportionately increased dissociation constants when glutathionylated. This defines a key role for glutathionylation in superoxide dismutase 1 associated familial amyotrophic lateral sclerosis.

Publication Date


  • 2013

Citation


  • McAlary, L., Yerbury, J. J. & Aquilina, J. (2013). Glutathionylation potentiates benign superoxide dismutase 1 variants to the toxic forms associated with amyotrophic lateral sclerosis. Scientific Reports, 3 3275-1-3275-6.

Scopus Eid


  • 2-s2.0-84888218077

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2249&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1231

Start Page


  • 3275-1

End Page


  • 3275-6

Volume


  • 3