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GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile

Journal Article


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Abstract


  • We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul A.
  •   Pyne, Stephen G.
  •   Taweechotipatr, Malai (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Taweechotipatr, M. & Kamchonwongpaisan, S. (2013). GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications: an international journal for communications and reviews, 8 (9), 1305-1308.

Scopus Eid


  • 2-s2.0-84886805648

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2271&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1253

Has Global Citation Frequency


Number Of Pages


  • 3

Start Page


  • 1305

End Page


  • 1308

Volume


  • 8

Issue


  • 9

Place Of Publication


  • United States

Abstract


  • We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with (E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; (E)-isomyristicin (1), (E)-isoapiol (2), methyl eugenol (3) and (E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul A.
  •   Pyne, Stephen G.
  •   Taweechotipatr, Malai (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Taweechotipatr, M. & Kamchonwongpaisan, S. (2013). GC/GC-MS analysis, isolation and identification of bioactive essential oil components from the Bhutanese medicinal plant, Pleurospermum amabile. Natural Product Communications: an international journal for communications and reviews, 8 (9), 1305-1308.

Scopus Eid


  • 2-s2.0-84886805648

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2271&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1253

Has Global Citation Frequency


Number Of Pages


  • 3

Start Page


  • 1305

End Page


  • 1308

Volume


  • 8

Issue


  • 9

Place Of Publication


  • United States