Skip to main content

Bionic materials for neuromuscular restoration and maintenance

Journal Article


Abstract


  • Effective engineering of skeletal muscle

    requires platforms that facilitate the

    proliferation and maintenance of primary

    muscle stem cells (myoblasts) and muscle

    fibre maturation in a manner that reflects

    native muscle structure. In addition, the

    supporting scaffold needs to accommodate the

    correct innervation of the re-engineered

    muscle tissue by promoting axonal connection

    and neuromuscular junction formation. We

    have been investigating the use of micro and

    nano-structured conducting polymer surfaces

    for ex vivo muscle and nerve growth,

    differentiation and trophic electrical

    stimulation. Micro-structured platforms were

    created by localization of wet-spun

    PLA:PLGA fibers onto polypyrrole substrate,

    whilst nano-structured platforms were created

    by orientation of carbon nanotube fibres on a

    conducting gold mylar surface, over which a

    layers of polypyrrole were deposited. Human

    and murine myoblasts and rat dorsal root

    ganglion explants (sensory nerve) were grown

    and/or differentiated on these platforms. A

    significant effect on myotube orientation was

    seen on both micro and nano-structured

    surfaces whilst surface topography similarly

    influenced the direction in which elements of

    the DRG cellular components grew. Growth

    of muscle cells as well as sensory nerve

    components (Scwhann cells and axons) on

    both nano and micro-structured polypyrrole

    was increased by electrical stimulation,

    providing a novel model system by which the

    effective innervation of regenerating muscle

    can be explored.

Publication Date


  • 2013

Citation


  • Kapsa, R., Quigley, A. F., Kita, M., Mysore, T., Moulton, S., Higgins, M. & Wallace, G. G. (2013). Bionic materials for neuromuscular restoration and maintenance. Journal of Gene Medicine, 15 (8-9), 324-324.

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/947

Number Of Pages


  • 0

Start Page


  • 324

End Page


  • 324

Volume


  • 15

Issue


  • 8-9

Place Of Publication


  • United States

Abstract


  • Effective engineering of skeletal muscle

    requires platforms that facilitate the

    proliferation and maintenance of primary

    muscle stem cells (myoblasts) and muscle

    fibre maturation in a manner that reflects

    native muscle structure. In addition, the

    supporting scaffold needs to accommodate the

    correct innervation of the re-engineered

    muscle tissue by promoting axonal connection

    and neuromuscular junction formation. We

    have been investigating the use of micro and

    nano-structured conducting polymer surfaces

    for ex vivo muscle and nerve growth,

    differentiation and trophic electrical

    stimulation. Micro-structured platforms were

    created by localization of wet-spun

    PLA:PLGA fibers onto polypyrrole substrate,

    whilst nano-structured platforms were created

    by orientation of carbon nanotube fibres on a

    conducting gold mylar surface, over which a

    layers of polypyrrole were deposited. Human

    and murine myoblasts and rat dorsal root

    ganglion explants (sensory nerve) were grown

    and/or differentiated on these platforms. A

    significant effect on myotube orientation was

    seen on both micro and nano-structured

    surfaces whilst surface topography similarly

    influenced the direction in which elements of

    the DRG cellular components grew. Growth

    of muscle cells as well as sensory nerve

    components (Scwhann cells and axons) on

    both nano and micro-structured polypyrrole

    was increased by electrical stimulation,

    providing a novel model system by which the

    effective innervation of regenerating muscle

    can be explored.

Publication Date


  • 2013

Citation


  • Kapsa, R., Quigley, A. F., Kita, M., Mysore, T., Moulton, S., Higgins, M. & Wallace, G. G. (2013). Bionic materials for neuromuscular restoration and maintenance. Journal of Gene Medicine, 15 (8-9), 324-324.

Ro Metadata Url


  • http://ro.uow.edu.au/aiimpapers/947

Number Of Pages


  • 0

Start Page


  • 324

End Page


  • 324

Volume


  • 15

Issue


  • 8-9

Place Of Publication


  • United States