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Artificial fertilisation in a terrestrial toadlet (Pseudophryne guentheri): effect of medium osmolality, sperm concentration and gamete storage

Journal Article


Abstract


  • Anurans exhibit a greater reproductive diversity than any other vertebrate order. However, studies investigating the effects of the external fertilisation environment on fertilisation success are limited to aquatic-breeding species. This study investigated the effects of fertilisation medium osmolality, sperm concentration and short-term oocyte storage on fertilisation success in a terrestrial-breeding anuran, Pseudophryne guentheri. Split-clutch experimental designs were used to determine optimal fertilisation conditions. To determine the effect of short-term sperm storage, sperm viability was assessed using fluorescence microscopy and percentage sperm motility and velocity quantified with a computer-assisted sperm analysis system. Fertilisation success was highest in media ranging in osmolality from 25mOsmkg-1 to 100mOsmkg-1, representing a broader range and higher optimal osmolality than previously reported for aquatic breeders. High rates of fertilisation (>75%) were achieved in relatively low sperm concentrations (2.5×104mL-1). Oocytes stored in isotonic solutions (200mOsmkg-1) retained fertilisation capacity (32%) after 8h of storage, while sperm suspensions maintained motility (≥26%) for 13 days. Additional studies on terrestrial-breeding anurans will be required to ascertain whether the optimal fertilisation conditions reported reflect adaptations to achieve fertilisation in a terrestrial environment.

Publication Date


  • 2013

Citation


  • Silla, A. J. (2013). Artificial fertilisation in a terrestrial toadlet (Pseudophryne guentheri): effect of medium osmolality, sperm concentration and gamete storage. Reproduction Fertility and Development, 25 (8), 1134-1141.

Scopus Eid


  • 2-s2.0-84885614150

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1250

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1134

End Page


  • 1141

Volume


  • 25

Issue


  • 8

Place Of Publication


  • Australia

Abstract


  • Anurans exhibit a greater reproductive diversity than any other vertebrate order. However, studies investigating the effects of the external fertilisation environment on fertilisation success are limited to aquatic-breeding species. This study investigated the effects of fertilisation medium osmolality, sperm concentration and short-term oocyte storage on fertilisation success in a terrestrial-breeding anuran, Pseudophryne guentheri. Split-clutch experimental designs were used to determine optimal fertilisation conditions. To determine the effect of short-term sperm storage, sperm viability was assessed using fluorescence microscopy and percentage sperm motility and velocity quantified with a computer-assisted sperm analysis system. Fertilisation success was highest in media ranging in osmolality from 25mOsmkg-1 to 100mOsmkg-1, representing a broader range and higher optimal osmolality than previously reported for aquatic breeders. High rates of fertilisation (>75%) were achieved in relatively low sperm concentrations (2.5×104mL-1). Oocytes stored in isotonic solutions (200mOsmkg-1) retained fertilisation capacity (32%) after 8h of storage, while sperm suspensions maintained motility (≥26%) for 13 days. Additional studies on terrestrial-breeding anurans will be required to ascertain whether the optimal fertilisation conditions reported reflect adaptations to achieve fertilisation in a terrestrial environment.

Publication Date


  • 2013

Citation


  • Silla, A. J. (2013). Artificial fertilisation in a terrestrial toadlet (Pseudophryne guentheri): effect of medium osmolality, sperm concentration and gamete storage. Reproduction Fertility and Development, 25 (8), 1134-1141.

Scopus Eid


  • 2-s2.0-84885614150

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1250

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 1134

End Page


  • 1141

Volume


  • 25

Issue


  • 8

Place Of Publication


  • Australia