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Autologous pump-perfused rat hind limb preparation for investigating muscle function and metabolism in vivo

Journal Article


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Abstract


  • Objective

    Oxygen delivery, underpinned by vascular tone, is the principle limiting factor in the study of skeletal muscle physiology, particularly during muscle contraction. The aim of this study was to develop an autologous perfused rat hind limb preparation for the study of skeletal muscle contractile function.

    Methods

    Adult Wistar rats were surgically prepared using a by-pass system for pump-controlled arterial blood flow to, and venous return from the hind limb during periods of quiescence and twitch contraction of the gastrocnemius-plantaris-soleus muscle bundle.

    Results

    During rest, hind limb perfusion pressure (102 ± 5 mmHg) was not different to systemic arterial pressure (99 ± 4 mmHg). Hind limb pressure was responsive to vasoconstrictors and vasodilators (±50 mmHg). The arterial PO2 (100 ± 3 mmHg), O2 saturation, and acid–base balance (pH: 7.42 ± 0.01) contributed to resting hind limb (a-v)O2 difference (4.8 ± 0.5 mL/100 mL) and VO2 (0.31 ± 0.03 μmol/g/min wet weight). Repetitive isometric twitch tension (1 Hz, 0.05 ms, 10 minutes) was best maintained at a flow rate of 2 mL/min (VO2 increased fivefold during muscle contraction) and efficiency of oxygen use increased from 0.27 ± 0.08–0.52 ± 0.07 N/μmol/min.

    Conclusion

    The autologous rat hind limb provided resting vascular tone allowing maintenance of perfusion pressure at flows within the physiological range. Oxygen delivery supported repetitive twitch contractions and facilitated measurement of active metabolism.

UOW Authors


  •   Peoples, Gregory
  •   Hoy, Andrew J. (external author)
  •   Henry, Renee (external author)
  •   McLennan, Peter (external author)

Publication Date


  • 2013

Citation


  • Peoples, G. E., Hoy, A. J., Henry, R. & McLennan, P. L. (2013). Autologous pump-perfused rat hind limb preparation for investigating muscle function and metabolism in vivo. Microcirculation, 20 (6), 511-523.

Scopus Eid


  • 2-s2.0-84881500708

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2042&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1024

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 511

End Page


  • 523

Volume


  • 20

Issue


  • 6

Place Of Publication


  • United Kingdom

Abstract


  • Objective

    Oxygen delivery, underpinned by vascular tone, is the principle limiting factor in the study of skeletal muscle physiology, particularly during muscle contraction. The aim of this study was to develop an autologous perfused rat hind limb preparation for the study of skeletal muscle contractile function.

    Methods

    Adult Wistar rats were surgically prepared using a by-pass system for pump-controlled arterial blood flow to, and venous return from the hind limb during periods of quiescence and twitch contraction of the gastrocnemius-plantaris-soleus muscle bundle.

    Results

    During rest, hind limb perfusion pressure (102 ± 5 mmHg) was not different to systemic arterial pressure (99 ± 4 mmHg). Hind limb pressure was responsive to vasoconstrictors and vasodilators (±50 mmHg). The arterial PO2 (100 ± 3 mmHg), O2 saturation, and acid–base balance (pH: 7.42 ± 0.01) contributed to resting hind limb (a-v)O2 difference (4.8 ± 0.5 mL/100 mL) and VO2 (0.31 ± 0.03 μmol/g/min wet weight). Repetitive isometric twitch tension (1 Hz, 0.05 ms, 10 minutes) was best maintained at a flow rate of 2 mL/min (VO2 increased fivefold during muscle contraction) and efficiency of oxygen use increased from 0.27 ± 0.08–0.52 ± 0.07 N/μmol/min.

    Conclusion

    The autologous rat hind limb provided resting vascular tone allowing maintenance of perfusion pressure at flows within the physiological range. Oxygen delivery supported repetitive twitch contractions and facilitated measurement of active metabolism.

UOW Authors


  •   Peoples, Gregory
  •   Hoy, Andrew J. (external author)
  •   Henry, Renee (external author)
  •   McLennan, Peter (external author)

Publication Date


  • 2013

Citation


  • Peoples, G. E., Hoy, A. J., Henry, R. & McLennan, P. L. (2013). Autologous pump-perfused rat hind limb preparation for investigating muscle function and metabolism in vivo. Microcirculation, 20 (6), 511-523.

Scopus Eid


  • 2-s2.0-84881500708

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2042&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1024

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 511

End Page


  • 523

Volume


  • 20

Issue


  • 6

Place Of Publication


  • United Kingdom