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Teasaponin improves central leptin sensitivity in high-fat diet-induced obese mice

Conference Paper


Abstract


  • Purpose: Leptin promotes negative energy balance by suppressing

    energy intake (EI) and elevating energy expenditure through its central

    action. Central leptin resistance is a hallmark of diet-induced obesity

    (DIO). Oral administration of teasaponin (TS) can reduce body weight

    and fat mass. However, little is known if TS has benefit effect in improving

    central leptin sensitivity. This study investigated the effects of TS on

    central leptin sensitivity and leptin signalling in the hypothalamic arcuate

    nucleus (Arc) of DIO mice. Methods: After 15 weeks of high-fat diet, DIO

    mice (n=40) were divided into two groups received either intraperitoneal

    (ip) injection of TS (10mg/kg, daily) or saline for 20 days. Another group

    of mice was fed low-fat diet (LF) as control (n=20). Then both DIO mice

    and LF mice were given intracerebroventricular (i.c.v.) injection of leptin

    or saline. Food intake and pSTAT3 level in the Arc in response to central

    leptin administration were assessed. Results: TS significantly decreased

    body weight gain (-210%, p<0.001) and average EI (-24%, p<0.001) of

    DIO mice. In LF mice, i.c.v. injection of leptin significantly suppressed food

    intake compared to saline injection (-29%, p=0.001). However, central

    leptin sensitivity was blunted in DIO mice evidenced by an incapable

    of suppressing food intake compared to control mice. Importantly, TS

    treatment reinstalled leptin sensitivity as seeing a significant decrease

    in food intake after i.c.v. leptin injection for 24 hours (-39%, p=0.023).

    With TS treatment, pSTAT3 level in the Arc also increased in response

    to central leptin injection compared to saline group. Conclusion: This

    study suggested that teasaponin can correct central leptin resistance in

    DIO mice via improving leptin-pSTAT3 signalling in the hypothalamus.

Authors


Publication Date


  • 2013

Citation


  • Wu, Y., Huang, X. F. & Yu, Y. (2013). Teasaponin improves central leptin sensitivity in high-fat diet-induced obese mice. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 161-161). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/909

Start Page


  • 161

End Page


  • 161

Place Of Publication


  • Australia

Abstract


  • Purpose: Leptin promotes negative energy balance by suppressing

    energy intake (EI) and elevating energy expenditure through its central

    action. Central leptin resistance is a hallmark of diet-induced obesity

    (DIO). Oral administration of teasaponin (TS) can reduce body weight

    and fat mass. However, little is known if TS has benefit effect in improving

    central leptin sensitivity. This study investigated the effects of TS on

    central leptin sensitivity and leptin signalling in the hypothalamic arcuate

    nucleus (Arc) of DIO mice. Methods: After 15 weeks of high-fat diet, DIO

    mice (n=40) were divided into two groups received either intraperitoneal

    (ip) injection of TS (10mg/kg, daily) or saline for 20 days. Another group

    of mice was fed low-fat diet (LF) as control (n=20). Then both DIO mice

    and LF mice were given intracerebroventricular (i.c.v.) injection of leptin

    or saline. Food intake and pSTAT3 level in the Arc in response to central

    leptin administration were assessed. Results: TS significantly decreased

    body weight gain (-210%, p<0.001) and average EI (-24%, p<0.001) of

    DIO mice. In LF mice, i.c.v. injection of leptin significantly suppressed food

    intake compared to saline injection (-29%, p=0.001). However, central

    leptin sensitivity was blunted in DIO mice evidenced by an incapable

    of suppressing food intake compared to control mice. Importantly, TS

    treatment reinstalled leptin sensitivity as seeing a significant decrease

    in food intake after i.c.v. leptin injection for 24 hours (-39%, p=0.023).

    With TS treatment, pSTAT3 level in the Arc also increased in response

    to central leptin injection compared to saline group. Conclusion: This

    study suggested that teasaponin can correct central leptin resistance in

    DIO mice via improving leptin-pSTAT3 signalling in the hypothalamus.

Authors


Publication Date


  • 2013

Citation


  • Wu, Y., Huang, X. F. & Yu, Y. (2013). Teasaponin improves central leptin sensitivity in high-fat diet-induced obese mice. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 161-161). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/909

Start Page


  • 161

End Page


  • 161

Place Of Publication


  • Australia