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Antioxidant properties of Apolipoprotein-D methionine- and selenomethioninecontaining peptides

Conference Paper


Abstract


  • Apolipoprotein D (ApoD), a lipocalin abundantly present in the central

    nervous system, has been recently shown to combat oxidative stress.

    This protein increases expression during Alzheimer’s disease and other

    neurodegenerative diseases, indicating that this is one of the body’s

    natural defences against redox events. The reducing capacity of ApoD

    is believed to come from Met93, which is exposed within the ligandbinding

    region of the protein. Purpose: We have developed nine peptides

    based on the three methionines within ApoD to determine the strength

    of reduction caused by methionine, and whether this is affected by the

    surrounding amino acids. In addition, selenomethionine mutants have

    been used in order to establish whether selenium confers a stronger

    redox capability than sulfur, and whether peptides containing this amino

    acid can be recycled to perpetuate the antioxidant cycle. Results:

    Initial studies have proved Met49 and SeMet49 to be highly successful

    reductants, with the SeMet49 peptide demonstrating extremely rapid

    reduction within the HPLC studies, as well as in experiments using

    liposomes as a model for membrane lipids. The SeMet93 is capable of

    bringing the peroxidation of liposomes to baseline level over a 12 hour

    timecourse.

Publication Date


  • 2013

Citation


  • Kornfeld, S. M. & Garner, B. (2013). Antioxidant properties of Apolipoprotein-D methionine- and selenomethioninecontaining peptides. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 124-124). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/907

Start Page


  • 124

End Page


  • 124

Abstract


  • Apolipoprotein D (ApoD), a lipocalin abundantly present in the central

    nervous system, has been recently shown to combat oxidative stress.

    This protein increases expression during Alzheimer’s disease and other

    neurodegenerative diseases, indicating that this is one of the body’s

    natural defences against redox events. The reducing capacity of ApoD

    is believed to come from Met93, which is exposed within the ligandbinding

    region of the protein. Purpose: We have developed nine peptides

    based on the three methionines within ApoD to determine the strength

    of reduction caused by methionine, and whether this is affected by the

    surrounding amino acids. In addition, selenomethionine mutants have

    been used in order to establish whether selenium confers a stronger

    redox capability than sulfur, and whether peptides containing this amino

    acid can be recycled to perpetuate the antioxidant cycle. Results:

    Initial studies have proved Met49 and SeMet49 to be highly successful

    reductants, with the SeMet49 peptide demonstrating extremely rapid

    reduction within the HPLC studies, as well as in experiments using

    liposomes as a model for membrane lipids. The SeMet93 is capable of

    bringing the peroxidation of liposomes to baseline level over a 12 hour

    timecourse.

Publication Date


  • 2013

Citation


  • Kornfeld, S. M. & Garner, B. (2013). Antioxidant properties of Apolipoprotein-D methionine- and selenomethioninecontaining peptides. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 124-124). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/907

Start Page


  • 124

End Page


  • 124