Purpose: Recently, studies have shown that pentacyclic triterpenes have
anti-inflammatory effects in the brains of mice with obesity; a disorder
where inflammation largely contributes to energy balance dysregulation.
The purpose of this study was to: 1) Investigate for the first time if the
oleanolic acid (OA) derivative, bardoxolone methyl (BM) has a more
potent effect than ursolic acid (UA) and OA in reducing body weight in
mice fed a high fat diet (HFD) for 10 weeks; 2) To analyse hypothalamic
tissue of mice in this study to determine if UA, OA and BM improve
key neuronal molecules involved in energy expenditure. Methods:
12 week C57B1/6 male mice (n=70) were divided into 5 groups (low
fat diet (LFD), HFD, HFD + UA, HFD + OA and HFD +BM). BM, UA
and OA were administered in drinking water at a dosage of 10mg/kg.
Signalling molecules involved in energy expenditure is being analysed
using western blotting and Real time polymerase chain reaction (RTPCR).
One and two way analysis of variance (ANOVA) and post-hoc
Tukey-HSD test were performed for statistical analysis. Results: BM
significantly prevented body weight gain in mice fed a HFD compared to
the mice treated with OA and UA (p≤<0.05). Furthermore, BM reduced
body weight (p≤<0.05). No differences in energy and water intake
among the HFD groups have been found (p≥<0.05). The experiment is
currently in progress and further data will be presented at the conference.
Conclusion: The preliminary data have shown that BM significantly
reduces body weight gain in mice fed a HFD (p≤<0.05). These results
indicate that BM induced negative energy balance is influenced by the
energy expenditure pathway of neuronal networks.