Skip to main content
placeholder image

Central administrations of palmitic acid and arachidonic acid decrease central leptin sensitivity in mice

Conference Paper


Abstract


  • Purpose: Leptin inhibits feeding and increases energy expenditure

    through the central nervous system. High-fat diet with saturated fatty

    acids (SFA) or n-6 polyunsaturated fatty acids (n-6 PUFA) has been

    reported to induce central leptin resistance and obesity. However, little

    is known if central administration of SFA or n-6 PUFA can reduce central

    leptin sensitivity. This study examined the central leptin sensitivity in

    response to intracerebroventricular (i.c.v.) injection of SFA, palmitic acid

    (PA) and n-6 PUFA, arachidonic acid (ARA) in mice. Methods: After

    overnight fasting, C57BL/6J male mice (n=24/group) were i.c.v. injected

    with either PA (50pmol/2ul), ARA (50pmol/2ul) or vehicle (saline, 2ul) prior

    to i.c.v. injection of leptin (0.5ug/2ul) or saline followed by refeeding for

    24 hours ad libitum on normal diet. Food intake was measured at 1, 4, 16

    and 24 hours after leptin i.c.v. injection. Body weight gain was measured

    24 hours after leptin injection. Results: PA and ARA icv injection did not

    significantly change food intake in mice compared with vehicle group.

    In vehicle group, central leptin administration significantly suppressed

    food intake at 1hour (64.60%, p=0.001), 4hour (54.98%, p=0.003),

    16hour (54.34%, p<0.001) and 24hour (52.72%, p<0.001). In PA and

    ARA pre-treatment group, leptin did not significantly suppress food

    intake compared with saline injection at 16 and 24 hour. Furthermore,

    leptin significantly decreased body weight gain for 24 hours in vehicle

    group (404.69%, p<0.001). However, in ARA pre-treatment group, there

    was no significant difference in the body weight gain after central leptin

    and saline injection. Conclusion: This study suggested that central

    administration of saturated fatty acid PA and n-6 unsaturated fatty acid

    ARA could induce central leptin resistance.

Publication Date


  • 2013

Citation


  • Cheng, L., Huang, X. & Yu, Y. (2013). Central administrations of palmitic acid and arachidonic acid decrease central leptin sensitivity in mice. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 96-96). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/900

Start Page


  • 96

End Page


  • 96

Place Of Publication


  • Australia

Abstract


  • Purpose: Leptin inhibits feeding and increases energy expenditure

    through the central nervous system. High-fat diet with saturated fatty

    acids (SFA) or n-6 polyunsaturated fatty acids (n-6 PUFA) has been

    reported to induce central leptin resistance and obesity. However, little

    is known if central administration of SFA or n-6 PUFA can reduce central

    leptin sensitivity. This study examined the central leptin sensitivity in

    response to intracerebroventricular (i.c.v.) injection of SFA, palmitic acid

    (PA) and n-6 PUFA, arachidonic acid (ARA) in mice. Methods: After

    overnight fasting, C57BL/6J male mice (n=24/group) were i.c.v. injected

    with either PA (50pmol/2ul), ARA (50pmol/2ul) or vehicle (saline, 2ul) prior

    to i.c.v. injection of leptin (0.5ug/2ul) or saline followed by refeeding for

    24 hours ad libitum on normal diet. Food intake was measured at 1, 4, 16

    and 24 hours after leptin i.c.v. injection. Body weight gain was measured

    24 hours after leptin injection. Results: PA and ARA icv injection did not

    significantly change food intake in mice compared with vehicle group.

    In vehicle group, central leptin administration significantly suppressed

    food intake at 1hour (64.60%, p=0.001), 4hour (54.98%, p=0.003),

    16hour (54.34%, p<0.001) and 24hour (52.72%, p<0.001). In PA and

    ARA pre-treatment group, leptin did not significantly suppress food

    intake compared with saline injection at 16 and 24 hour. Furthermore,

    leptin significantly decreased body weight gain for 24 hours in vehicle

    group (404.69%, p<0.001). However, in ARA pre-treatment group, there

    was no significant difference in the body weight gain after central leptin

    and saline injection. Conclusion: This study suggested that central

    administration of saturated fatty acid PA and n-6 unsaturated fatty acid

    ARA could induce central leptin resistance.

Publication Date


  • 2013

Citation


  • Cheng, L., Huang, X. & Yu, Y. (2013). Central administrations of palmitic acid and arachidonic acid decrease central leptin sensitivity in mice. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 96-96). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/900

Start Page


  • 96

End Page


  • 96

Place Of Publication


  • Australia