Skip to main content
placeholder image

Leptin signalling in the dorsal vagal complex and ventrolateral medulla with age and high-fat diet induced obesity

Conference Paper


Abstract


  • Purpose: The brainstem is a known contributor to central energy

    regulation and responds to leptin. Currently, the leptin signalling response

    to high-fat diet induced obesity in the brainstem is poorly understood.

    In this study we examined key leptin signalling markers in the Dorsal

    Vagal Complex (DVC) and Ventrolateral Medulla (VLM) of mice during

    mid-term (8 weeks) and long-term (20 weeks) high-fat diet induced

    obesity. Methods: C57Bl/6 male mice aged 12 weeks were fed either

    a high-fat (HF) or a low-fat control diet (LF), then examined after 8 or

    20 weeks of feeding. Leptin signalling markers in the brainstem were

    examined by Western blot (n=3-8) following the intracerebroventricular

    injection of leptin or saline. Results: In the DVC, central leptin injection

    significantly increased pSTAT3 levels in the 8 week LF group (p=0.021),

    but not in the HF group. However, leptin administration did not increase

    pSTAT3 levels in either 20 week LF or HF group. In the VLM, pSTAT3

    was significantly increased in the 8 week HF group compared with the

    LF group (p=0.038). pAMPK in the VLM decreased (p=0.023), while

    POMC in the DVC increased (p=0.049), in response to leptin injection

    in the 20 week LF group, but not in the 8 week LF group. Conclusion:

    Both high-fat diet and age impede leptin-pSTAT3 signalling in the DVC

    of the brainstem. Mid-term high-fat diet can activate pSTAT3 in the

    VLM. Age associated leptin-pSTAT3 deficiency in the brainstem may be

    compensated for by decreased pAMPK signalling and increased POMC

    expression in response to central leptin.

Authors


  •   Bell, Christopher (external author)
  •   Yu, Yinghua
  •   Szabo, Alexander M. (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2013

Citation


  • Bell, C., Yu, Y. H., Szabo, A. & Huang, X. F. (2013). Leptin signalling in the dorsal vagal complex and ventrolateral medulla with age and high-fat diet induced obesity. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 35-35). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/896

Start Page


  • 35

End Page


  • 35

Place Of Publication


  • Australia

Abstract


  • Purpose: The brainstem is a known contributor to central energy

    regulation and responds to leptin. Currently, the leptin signalling response

    to high-fat diet induced obesity in the brainstem is poorly understood.

    In this study we examined key leptin signalling markers in the Dorsal

    Vagal Complex (DVC) and Ventrolateral Medulla (VLM) of mice during

    mid-term (8 weeks) and long-term (20 weeks) high-fat diet induced

    obesity. Methods: C57Bl/6 male mice aged 12 weeks were fed either

    a high-fat (HF) or a low-fat control diet (LF), then examined after 8 or

    20 weeks of feeding. Leptin signalling markers in the brainstem were

    examined by Western blot (n=3-8) following the intracerebroventricular

    injection of leptin or saline. Results: In the DVC, central leptin injection

    significantly increased pSTAT3 levels in the 8 week LF group (p=0.021),

    but not in the HF group. However, leptin administration did not increase

    pSTAT3 levels in either 20 week LF or HF group. In the VLM, pSTAT3

    was significantly increased in the 8 week HF group compared with the

    LF group (p=0.038). pAMPK in the VLM decreased (p=0.023), while

    POMC in the DVC increased (p=0.049), in response to leptin injection

    in the 20 week LF group, but not in the 8 week LF group. Conclusion:

    Both high-fat diet and age impede leptin-pSTAT3 signalling in the DVC

    of the brainstem. Mid-term high-fat diet can activate pSTAT3 in the

    VLM. Age associated leptin-pSTAT3 deficiency in the brainstem may be

    compensated for by decreased pAMPK signalling and increased POMC

    expression in response to central leptin.

Authors


  •   Bell, Christopher (external author)
  •   Yu, Yinghua
  •   Szabo, Alexander M. (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2013

Citation


  • Bell, C., Yu, Y. H., Szabo, A. & Huang, X. F. (2013). Leptin signalling in the dorsal vagal complex and ventrolateral medulla with age and high-fat diet induced obesity. 33rd Meeting of the Australian Neuroscience Society: Program, Abstracts & List of Registrants (pp. 35-35). Australia: ANS.

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/896

Start Page


  • 35

End Page


  • 35

Place Of Publication


  • Australia