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Antimicrobial, antimalarial and cytoxicity activities of constituents of a Bhutanese variety of Ajania nubigena

Journal Article


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Abstract


  • An investigation of the essential oil (EO) and the crude MeOH extract of a Bhutanese variety of Ajania nubigena using GC/GC-MS and NMR found the

    following: a) one kg of the dried plant material contained 0.7% w/w EO; b) 44 of the 53 GC-FID peaks of the EO were identified with (3R,6R)-linalool oxide

    acetate (75.8 %) as the major constituent (chemotype II) and chamazulene as a new sub-chemotype; c) purification of the EO furnished (3R,6R)-linalool oxide

    acetate (1), chamazulene (2), (E)-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3), and (Z)-2-(2,4-hexadinylidene)-1,6-dioxaspiro[4,4]non-3-ene (4);

    d) from the crude MeOH extract, four flavonoid compounds: 1-(4-hydroxyphenyl)propan-1-one (5), oxyanin B (6), luteolin (7) (major) and the luteolin-7-O--

    D-glucoside (8) were isolated; e) among the EO and pure compounds tested for biological activities, compound 7 exhibited a broad range of moderate

    antiplasmodial, cytoxicity and antimicrobial activities; c) compound 8 showed significant in vitro antiplasmodial activity against P. falciparum strains TM4/8.2

    and K1CB1 (multidrug resistant strain) and was identified as a potential antimalarial scaffold; and f) the in vitro antimicrobial and cytotoxicity activities were

    in alignment with the traditional medical uses of this plant and thus substantiate its use in Bhutanese traditional medicine.

UOW Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul
  •   Pyne, Stephen
  •   Korth, John (external author)
  •   Samten, - (external author)
  •   Taweechotipatr, Malai (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Korth, J., Samten, , Taweechotipatr, M., Rattanajak, R. & Kamchonwongpaisan, S. (2013). Antimicrobial, antimalarial and cytoxicity activities of constituents of a Bhutanese variety of Ajania nubigena. Natural Product Communications: an international journal for communications and reviews, 8 (6), 733-736.

Scopus Eid


  • 2-s2.0-84886406698

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2254&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1236

Number Of Pages


  • 3

Start Page


  • 733

End Page


  • 736

Volume


  • 8

Issue


  • 6

Abstract


  • An investigation of the essential oil (EO) and the crude MeOH extract of a Bhutanese variety of Ajania nubigena using GC/GC-MS and NMR found the

    following: a) one kg of the dried plant material contained 0.7% w/w EO; b) 44 of the 53 GC-FID peaks of the EO were identified with (3R,6R)-linalool oxide

    acetate (75.8 %) as the major constituent (chemotype II) and chamazulene as a new sub-chemotype; c) purification of the EO furnished (3R,6R)-linalool oxide

    acetate (1), chamazulene (2), (E)-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3), and (Z)-2-(2,4-hexadinylidene)-1,6-dioxaspiro[4,4]non-3-ene (4);

    d) from the crude MeOH extract, four flavonoid compounds: 1-(4-hydroxyphenyl)propan-1-one (5), oxyanin B (6), luteolin (7) (major) and the luteolin-7-O--

    D-glucoside (8) were isolated; e) among the EO and pure compounds tested for biological activities, compound 7 exhibited a broad range of moderate

    antiplasmodial, cytoxicity and antimicrobial activities; c) compound 8 showed significant in vitro antiplasmodial activity against P. falciparum strains TM4/8.2

    and K1CB1 (multidrug resistant strain) and was identified as a potential antimalarial scaffold; and f) the in vitro antimicrobial and cytotoxicity activities were

    in alignment with the traditional medical uses of this plant and thus substantiate its use in Bhutanese traditional medicine.

UOW Authors


  •   Wangchuk, Phurpa (external author)
  •   Keller, Paul
  •   Pyne, Stephen
  •   Korth, John (external author)
  •   Samten, - (external author)
  •   Taweechotipatr, Malai (external author)
  •   Rattanajak, Roonglawan (external author)
  •   Kamchonwongpaisan, Sumalee (external author)

Publication Date


  • 2013

Citation


  • Wangchuk, P., Keller, P. A., Pyne, S. G., Korth, J., Samten, , Taweechotipatr, M., Rattanajak, R. & Kamchonwongpaisan, S. (2013). Antimicrobial, antimalarial and cytoxicity activities of constituents of a Bhutanese variety of Ajania nubigena. Natural Product Communications: an international journal for communications and reviews, 8 (6), 733-736.

Scopus Eid


  • 2-s2.0-84886406698

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=2254&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/1236

Number Of Pages


  • 3

Start Page


  • 733

End Page


  • 736

Volume


  • 8

Issue


  • 6