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Thermoresponsive cellulosic hydrogels with cell-releasing behavior

Journal Article


Abstract


  • Here we report the preparation and characterization of thermoresponsive cellulosic hydrogels with cell-releasing behavior. Hydroxypropyl cellulose (HPC) was modified with methacrylic anhydride (MA). The resultant macromonomer, HPC-MA, retains the characteristic thermoresponsive phase behavior of HPC, with an onset temperature of 36 C and a lower critical solution temperature (LCST) of 37-38 C, as determined by turbidity measurement. Homogenous HPC-MA hydrogels were prepared by UV-cross-linking the aqueous solutions of the macromonomer at room temperature, and characterized by water contact angle and swelling ratio measurements, and dynamic mechanical analysis. These hydrogels exhibit temperature-dependent surface hydrophilicity and hydrophobicity, equilibrium water content as well as mechanical properties. Cell-releasing characteristics were demonstrated using African green monkey kidney cell line (COS-7 cells) and murine-derived embryonic stem cell line (Oct4b2). By reducing temperature to 4 C, the cultivated cells spontaneously detached from the hydrogels without the need of trypsin treatment. These unique properties make our HPC-MA hydrogels potential substrates for cell sheet engineering. © 2013 American Chemical Society.

UOW Authors


  •   Hoo, Siew Pei (external author)
  •   Sarvi, Fatemeh (external author)
  •   Li, Waiho (external author)
  •   Chan, Peggy P. Y. (external author)
  •   Yue, Zhilian

Publication Date


  • 2013

Citation


  • Hoo, S. P., Sarvi, F., Li, W. Ho., Chan, P. P. Y. & Yue, Z. (2013). Thermoresponsive cellulosic hydrogels with cell-releasing behavior. ACS Applied Materials and Interfaces, 5 (12), 5592-5600.

Scopus Eid


  • 2-s2.0-84879513624

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/1349

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 5592

End Page


  • 5600

Volume


  • 5

Issue


  • 12

Place Of Publication


  • United States

Abstract


  • Here we report the preparation and characterization of thermoresponsive cellulosic hydrogels with cell-releasing behavior. Hydroxypropyl cellulose (HPC) was modified with methacrylic anhydride (MA). The resultant macromonomer, HPC-MA, retains the characteristic thermoresponsive phase behavior of HPC, with an onset temperature of 36 C and a lower critical solution temperature (LCST) of 37-38 C, as determined by turbidity measurement. Homogenous HPC-MA hydrogels were prepared by UV-cross-linking the aqueous solutions of the macromonomer at room temperature, and characterized by water contact angle and swelling ratio measurements, and dynamic mechanical analysis. These hydrogels exhibit temperature-dependent surface hydrophilicity and hydrophobicity, equilibrium water content as well as mechanical properties. Cell-releasing characteristics were demonstrated using African green monkey kidney cell line (COS-7 cells) and murine-derived embryonic stem cell line (Oct4b2). By reducing temperature to 4 C, the cultivated cells spontaneously detached from the hydrogels without the need of trypsin treatment. These unique properties make our HPC-MA hydrogels potential substrates for cell sheet engineering. © 2013 American Chemical Society.

UOW Authors


  •   Hoo, Siew Pei (external author)
  •   Sarvi, Fatemeh (external author)
  •   Li, Waiho (external author)
  •   Chan, Peggy P. Y. (external author)
  •   Yue, Zhilian

Publication Date


  • 2013

Citation


  • Hoo, S. P., Sarvi, F., Li, W. Ho., Chan, P. P. Y. & Yue, Z. (2013). Thermoresponsive cellulosic hydrogels with cell-releasing behavior. ACS Applied Materials and Interfaces, 5 (12), 5592-5600.

Scopus Eid


  • 2-s2.0-84879513624

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/1349

Has Global Citation Frequency


Number Of Pages


  • 8

Start Page


  • 5592

End Page


  • 5600

Volume


  • 5

Issue


  • 12

Place Of Publication


  • United States