Bio-ink for on-demand printing of living cells

Journal Article


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Abstract


  • Drop-on-demand bioprinting allows the controlled placement of living cells, and will benefit research in the fields of tissue engineering, drug screening and toxicology. We show that a bio-ink based on a novel microgel suspension in a surfactant-containing tissue culture medium can be used to reproducibly print several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods. The bio-ink maintains a stable cell suspension, preventing the settling and aggregation of cells that usually impedes cell printing, whilst meeting the stringent fluid property requirements needed to enable printing even from many-nozzle commercial inkjet print heads. This innovation in printing technology may pave the way for the biofabrication of multi-cellular structures and functional tissue.

Publication Date


  • 2013

Citation


  • Ferris, C. J., Gilmore, K. J., Beirne, S., McCallum, D., Wallace, G. G. & in het Panhuis, M. (2013). Bio-ink for on-demand printing of living cells. Biomaterials Science, 1 (2), 224-230.

Scopus Eid


  • 2-s2.0-84875008763

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1579&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/566

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 224

End Page


  • 230

Volume


  • 1

Issue


  • 2

Place Of Publication


  • United Kingdom

Abstract


  • Drop-on-demand bioprinting allows the controlled placement of living cells, and will benefit research in the fields of tissue engineering, drug screening and toxicology. We show that a bio-ink based on a novel microgel suspension in a surfactant-containing tissue culture medium can be used to reproducibly print several different cell types, from two different commercially available drop-on-demand printing systems, over long printing periods. The bio-ink maintains a stable cell suspension, preventing the settling and aggregation of cells that usually impedes cell printing, whilst meeting the stringent fluid property requirements needed to enable printing even from many-nozzle commercial inkjet print heads. This innovation in printing technology may pave the way for the biofabrication of multi-cellular structures and functional tissue.

Publication Date


  • 2013

Citation


  • Ferris, C. J., Gilmore, K. J., Beirne, S., McCallum, D., Wallace, G. G. & in het Panhuis, M. (2013). Bio-ink for on-demand printing of living cells. Biomaterials Science, 1 (2), 224-230.

Scopus Eid


  • 2-s2.0-84875008763

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1579&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/566

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 224

End Page


  • 230

Volume


  • 1

Issue


  • 2

Place Of Publication


  • United Kingdom