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Microbeam radiation-induced tissue damage depends on the stage of vascular maturation

Journal Article


Abstract


  • Purpose: To explore the effects of microbeam radiation (MR) on vascular biology, we used the chick chorioallantoic

    membrane (CAM) model of an almost pure vascular system with immature vessels (lacking periendothelial

    coverage) at Day 8 and mature vessels (with coverage) at Day 12 of development.

    Methods and Materials: CAMs were irradiated with microplanar beams (width, 25 mm; interbeam spacing,

    200 mm) at entrance doses of 200 or 300 Gy and, for comparison, with a broad beam (seamless radiation

    [SLR]), with entrance doses of 5 to 40 Gy.

    Results: In vivo monitoring of Day-8 CAMvasculature 6 h after 200 GyMRrevealed a near total destruction of the

    immature capillary plexus. Conversely, 200 Gy MR barely affected Day-12 CAM mature microvasculature. Morphological

    evaluation of Day-12 CAMs after the dose was increased to 300 Gy revealed opened interendothelial

    junctions, which could explain the transient mesenchymal edema immediately after irradiation. Electron micrographs

    revealed cytoplasmic vacuolization of endothelial cells in the beam path, with disrupted luminal surfaces;

    often the lumen was engorged with erythrocytes and leukocytes. After 30 min, the capillary plexus adopted a striated

    metronomic pattern, with alternating destroyed and intact zones, corresponding to the beam and the interbeam

    paths within the array. SLR at a dose of 10 Gy caused growth retardation, resulting in a remarkable

    reduction in the vascular endpoint density 24 h postirradiation. A dose of 40 Gy damaged the entire CAM

    vasculature.

    Conclusions: The effects of MR are mediated by capillary damage, with tissue injury caused by insufficient blood

    supply. Vascular toxicity and physiological effects ofMRdepend on the stage of capillary maturation and appear in

    the first 15 to 60 min after irradiation. Conversely, the effects of SLR, due to the arrest of cell proliferation, persist

    for a longer time.

UOW Authors


  •   Brauer-Krisch, Elke (external author)
  •   Laissue, Jean Albert. (external author)
  •   Dr Alberto Bravin , Alberto (external author)
  •   Sabatasso, Sara (external author)
  •   Hlushchuk, Ruslan (external author)
  •   Graber, Werner (external author)
  •   Blattmann, Hans (external author)
  •   Gruber, Guenther (external author)
  •   Djonov, Valentin (external author)
  •   Corde, Stephanie B.

Publication Date


  • 2011

Geographic Focus


Citation


  • Sabatasso, S., Laissue, J. Albert., Hlushchuk, R., Graber, W., Bravin, A., Brauer-Krisch, E., corde, s., Blattmann, H., Gruber, G. & Djonov, V. (2011). Microbeam radiation-induced tissue damage depends on the stage of vascular maturation. International Journal of Radiation: Oncology - Biology - Physics, 80 (5), 1522-1532.

Scopus Eid


  • 2-s2.0-79960103753

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/542

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 1522

End Page


  • 1532

Volume


  • 80

Issue


  • 5

Abstract


  • Purpose: To explore the effects of microbeam radiation (MR) on vascular biology, we used the chick chorioallantoic

    membrane (CAM) model of an almost pure vascular system with immature vessels (lacking periendothelial

    coverage) at Day 8 and mature vessels (with coverage) at Day 12 of development.

    Methods and Materials: CAMs were irradiated with microplanar beams (width, 25 mm; interbeam spacing,

    200 mm) at entrance doses of 200 or 300 Gy and, for comparison, with a broad beam (seamless radiation

    [SLR]), with entrance doses of 5 to 40 Gy.

    Results: In vivo monitoring of Day-8 CAMvasculature 6 h after 200 GyMRrevealed a near total destruction of the

    immature capillary plexus. Conversely, 200 Gy MR barely affected Day-12 CAM mature microvasculature. Morphological

    evaluation of Day-12 CAMs after the dose was increased to 300 Gy revealed opened interendothelial

    junctions, which could explain the transient mesenchymal edema immediately after irradiation. Electron micrographs

    revealed cytoplasmic vacuolization of endothelial cells in the beam path, with disrupted luminal surfaces;

    often the lumen was engorged with erythrocytes and leukocytes. After 30 min, the capillary plexus adopted a striated

    metronomic pattern, with alternating destroyed and intact zones, corresponding to the beam and the interbeam

    paths within the array. SLR at a dose of 10 Gy caused growth retardation, resulting in a remarkable

    reduction in the vascular endpoint density 24 h postirradiation. A dose of 40 Gy damaged the entire CAM

    vasculature.

    Conclusions: The effects of MR are mediated by capillary damage, with tissue injury caused by insufficient blood

    supply. Vascular toxicity and physiological effects ofMRdepend on the stage of capillary maturation and appear in

    the first 15 to 60 min after irradiation. Conversely, the effects of SLR, due to the arrest of cell proliferation, persist

    for a longer time.

UOW Authors


  •   Brauer-Krisch, Elke (external author)
  •   Laissue, Jean Albert. (external author)
  •   Dr Alberto Bravin , Alberto (external author)
  •   Sabatasso, Sara (external author)
  •   Hlushchuk, Ruslan (external author)
  •   Graber, Werner (external author)
  •   Blattmann, Hans (external author)
  •   Gruber, Guenther (external author)
  •   Djonov, Valentin (external author)
  •   Corde, Stephanie B.

Publication Date


  • 2011

Geographic Focus


Citation


  • Sabatasso, S., Laissue, J. Albert., Hlushchuk, R., Graber, W., Bravin, A., Brauer-Krisch, E., corde, s., Blattmann, H., Gruber, G. & Djonov, V. (2011). Microbeam radiation-induced tissue damage depends on the stage of vascular maturation. International Journal of Radiation: Oncology - Biology - Physics, 80 (5), 1522-1532.

Scopus Eid


  • 2-s2.0-79960103753

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/542

Has Global Citation Frequency


Number Of Pages


  • 10

Start Page


  • 1522

End Page


  • 1532

Volume


  • 80

Issue


  • 5