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Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping

Journal Article


Abstract


  • This article presents a dielectrophoresis (DEP)-

    based microfluidic device with the three-dimensional (3D)

    microelectrode configuration for concentrating and separating

    particles in a continuous throughflow. The 3D electrode

    structure, where microelectrode array are patterned on

    both the top and bottom surfaces of the microchannel, is

    composed of three units: focusing, aligning and trapping.

    As particles flowing through the microfluidic channel, they

    are firstly focused and aligned by the funnel-shaped and

    parallel electrode array, respectively, before being captured

    at the trapping unit due to negative DEP force. For a mixture

    of two particle populations of different sizes or dielectric

    properties, with a careful selection of suspending medium

    and applied field, the population exhibits stronger negative

    DEP manipulated by the microelectrode array and, therefore,

    separated from the other population which is easily

    carried away toward the outlet due to hydrodynamic force.

    The functionality of the proposed microdevice was verified

    by concentrating different-sized polystyrene (PS) microparticles

    and yeast cells dynamically flowing in the

    microchannel. Moreover, separation based on size and

    dielectric properties was achieved by sorting PS microparticles,

    and isolating 5 lm PS particles from yeast cells,

    respectively. The performance of the proposed microconcentrator

    and separator was also studied, including the

    threshold voltage at which particles begin to be trapped,

    variation of cell-trapping efficiency with respect to the

    applied voltage and flow rate, and the efficiency of separation

    experiments. The proposed microdevice has various

    advantages, including multi-functionality, improved

    manipulation efficiency and throughput, easy fabrication

    and operation, etc., which shows a great potential for biological,

    chemical and medical applications.

Authors


  •   Li, Ming (external author)
  •   Li, Shunbo (external author)
  •   Cao, Wenbin (external author)
  •   Li, Weihua
  •   Wen, Weijia (external author)
  •   Alici, Gursel

Publication Date


  • 2013

Citation


  • Li, M., Li, S., Cao, W., Li, W., Wen, W. & Alici, G. (2013). Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping. Microfluidics and Nanofluidics, 14 (3-4), 527-539.

Scopus Eid


  • 2-s2.0-84878557501

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/489

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 527

End Page


  • 539

Volume


  • 14

Issue


  • 3-4

Place Of Publication


  • Germany

Abstract


  • This article presents a dielectrophoresis (DEP)-

    based microfluidic device with the three-dimensional (3D)

    microelectrode configuration for concentrating and separating

    particles in a continuous throughflow. The 3D electrode

    structure, where microelectrode array are patterned on

    both the top and bottom surfaces of the microchannel, is

    composed of three units: focusing, aligning and trapping.

    As particles flowing through the microfluidic channel, they

    are firstly focused and aligned by the funnel-shaped and

    parallel electrode array, respectively, before being captured

    at the trapping unit due to negative DEP force. For a mixture

    of two particle populations of different sizes or dielectric

    properties, with a careful selection of suspending medium

    and applied field, the population exhibits stronger negative

    DEP manipulated by the microelectrode array and, therefore,

    separated from the other population which is easily

    carried away toward the outlet due to hydrodynamic force.

    The functionality of the proposed microdevice was verified

    by concentrating different-sized polystyrene (PS) microparticles

    and yeast cells dynamically flowing in the

    microchannel. Moreover, separation based on size and

    dielectric properties was achieved by sorting PS microparticles,

    and isolating 5 lm PS particles from yeast cells,

    respectively. The performance of the proposed microconcentrator

    and separator was also studied, including the

    threshold voltage at which particles begin to be trapped,

    variation of cell-trapping efficiency with respect to the

    applied voltage and flow rate, and the efficiency of separation

    experiments. The proposed microdevice has various

    advantages, including multi-functionality, improved

    manipulation efficiency and throughput, easy fabrication

    and operation, etc., which shows a great potential for biological,

    chemical and medical applications.

Authors


  •   Li, Ming (external author)
  •   Li, Shunbo (external author)
  •   Cao, Wenbin (external author)
  •   Li, Weihua
  •   Wen, Weijia (external author)
  •   Alici, Gursel

Publication Date


  • 2013

Citation


  • Li, M., Li, S., Cao, W., Li, W., Wen, W. & Alici, G. (2013). Improved concentration and separation of particles in a 3D dielectrophoretic chip integrating focusing, aligning and trapping. Microfluidics and Nanofluidics, 14 (3-4), 527-539.

Scopus Eid


  • 2-s2.0-84878557501

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/489

Has Global Citation Frequency


Number Of Pages


  • 12

Start Page


  • 527

End Page


  • 539

Volume


  • 14

Issue


  • 3-4

Place Of Publication


  • Germany