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High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip

Journal Article


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Abstract


  • Integrating different steps on a chip for cell manipulations and sample preparation

    is of foremost importance to fully take advantage of microfluidic possibilities, and

    therefore make tests faster, cheaper and more accurate. We demonstrated particle

    manipulation in an integrated microfluidic device by applying hydrodynamic,

    electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) forces. The

    process involves generation of fluid flow by pressure difference, particle trapping

    by DEP force, and particle redirect by EO and EP forces. Both DC and AC signals

    were applied, taking advantages of DC EP, EO and AC DEP for on-chip particle

    manipulation. Since different types of particles respond differently to these signals,

    variations of DC and AC signals are capable to handle complex and highly variable

    colloidal and biological samples. The proposed technique can operate in a highthroughput

    manner with thirteen independent channels in radial directions for

    enrichment and separation in microfluidic chip. We evaluated our approach by

    collecting Polystyrene particles, yeast cells, and E. coli bacteria, which respond

    differently to electric field gradient. Live and dead yeast cells were separated

    successfully, validating the capability of our device to separate highly similar cells.

    Our results showed that this technique could achieve fast pre-concentration of

    colloidal particles and cells and separation of cells depending on their vitality.

    Hydrodynamic, DC electrophoretic and DC electroosmotic forces were used

    together instead of syringe pump to achieve sufficient fluid flow and particle

    mobility for particle trapping and sorting. By eliminating bulky mechanical pumps,

    this new technique has wide applications for in situ detection and analysis.

Authors


  •   Li, Shunbo (external author)
  •   Li, Ming (external author)
  •   Bougot-Robin, Kristelle (external author)
  •   Cao, Wenbin (external author)
  •   Chau, Irene Yeung. (external author)
  •   Li, Weihua
  •   Wen, Weijia (external author)

Publication Date


  • 2013

Citation


  • Li, S., Li, M., Bougot-Robin, K., Cao, W., Chau, I. Yeung Yeung., Li, W. & Wen, W. (2013). High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip. Biomicrofluidics, 7 (2), 024106-1-024106-14.

Scopus Eid


  • 2-s2.0-84877653169

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1492&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/487

Start Page


  • 024106-1

End Page


  • 024106-14

Volume


  • 7

Issue


  • 2

Abstract


  • Integrating different steps on a chip for cell manipulations and sample preparation

    is of foremost importance to fully take advantage of microfluidic possibilities, and

    therefore make tests faster, cheaper and more accurate. We demonstrated particle

    manipulation in an integrated microfluidic device by applying hydrodynamic,

    electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) forces. The

    process involves generation of fluid flow by pressure difference, particle trapping

    by DEP force, and particle redirect by EO and EP forces. Both DC and AC signals

    were applied, taking advantages of DC EP, EO and AC DEP for on-chip particle

    manipulation. Since different types of particles respond differently to these signals,

    variations of DC and AC signals are capable to handle complex and highly variable

    colloidal and biological samples. The proposed technique can operate in a highthroughput

    manner with thirteen independent channels in radial directions for

    enrichment and separation in microfluidic chip. We evaluated our approach by

    collecting Polystyrene particles, yeast cells, and E. coli bacteria, which respond

    differently to electric field gradient. Live and dead yeast cells were separated

    successfully, validating the capability of our device to separate highly similar cells.

    Our results showed that this technique could achieve fast pre-concentration of

    colloidal particles and cells and separation of cells depending on their vitality.

    Hydrodynamic, DC electrophoretic and DC electroosmotic forces were used

    together instead of syringe pump to achieve sufficient fluid flow and particle

    mobility for particle trapping and sorting. By eliminating bulky mechanical pumps,

    this new technique has wide applications for in situ detection and analysis.

Authors


  •   Li, Shunbo (external author)
  •   Li, Ming (external author)
  •   Bougot-Robin, Kristelle (external author)
  •   Cao, Wenbin (external author)
  •   Chau, Irene Yeung. (external author)
  •   Li, Weihua
  •   Wen, Weijia (external author)

Publication Date


  • 2013

Citation


  • Li, S., Li, M., Bougot-Robin, K., Cao, W., Chau, I. Yeung Yeung., Li, W. & Wen, W. (2013). High-throughput particle manipulation by hydrodynamic, electrokinetic, and dielectrophoretic effects in an integrated microfluidic chip. Biomicrofluidics, 7 (2), 024106-1-024106-14.

Scopus Eid


  • 2-s2.0-84877653169

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1492&context=eispapers

Ro Metadata Url


  • http://ro.uow.edu.au/eispapers/487

Start Page


  • 024106-1

End Page


  • 024106-14

Volume


  • 7

Issue


  • 2