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Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry

Journal Article


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Abstract


  • The deposition of biological material (biofouling) onto polymeric contact lenses is thought to be a major contributor to lens discomfort and hence discontinuation of wear. We describe a method to characterize lipid deposits directly from worn contact lenses utilizing liquid extraction surface analysis coupled to tandem mass spectrometry (LESA-MS/MS). This technique effected facile and reproducible extraction of lipids from the contact lens surfaces and identified lipid molecular species representing all major classes present in human tear film. Our data show that LESA-MS/MS is a rapid and comprehensive technique for the characterization of lipid-related biofouling on polymer surfaces.

UOW Authors


  •   Brown, Simon (external author)
  •   Huxtable, Liam H. (external author)
  •   Willcox, Mark D. P. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2013

Citation


  • Brown, S. H., Huxtable, L. H., Willcox, M. D. P., Blanksby, S. J. & Mitchell, T. W. (2013). Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry. The Analyst, 138 (5), 1316-1320.

Scopus Eid


  • 2-s2.0-84873664792

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1322&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/310

Number Of Pages


  • 4

Start Page


  • 1316

End Page


  • 1320

Volume


  • 138

Issue


  • 5

Abstract


  • The deposition of biological material (biofouling) onto polymeric contact lenses is thought to be a major contributor to lens discomfort and hence discontinuation of wear. We describe a method to characterize lipid deposits directly from worn contact lenses utilizing liquid extraction surface analysis coupled to tandem mass spectrometry (LESA-MS/MS). This technique effected facile and reproducible extraction of lipids from the contact lens surfaces and identified lipid molecular species representing all major classes present in human tear film. Our data show that LESA-MS/MS is a rapid and comprehensive technique for the characterization of lipid-related biofouling on polymer surfaces.

UOW Authors


  •   Brown, Simon (external author)
  •   Huxtable, Liam H. (external author)
  •   Willcox, Mark D. P. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2013

Citation


  • Brown, S. H., Huxtable, L. H., Willcox, M. D. P., Blanksby, S. J. & Mitchell, T. W. (2013). Automated surface sampling of lipids from worn contact lenses coupled with tandem mass spectrometry. The Analyst, 138 (5), 1316-1320.

Scopus Eid


  • 2-s2.0-84873664792

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1322&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/310

Number Of Pages


  • 4

Start Page


  • 1316

End Page


  • 1320

Volume


  • 138

Issue


  • 5