Direct lipid profiling of single cells from inkjet printed microarrays

Journal Article


Abstract


  • The on-demand printing of living cells using inkjet

    technologies has recently been demonstrated and allows for the controlled

    deposition of cells in microarrays. Here, we show that such arrays can be

    interrogated directly by robot-controlled liquid microextraction coupled with

    chip-based nanoelectospray mass spectrometry. Such automated analyses

    generate a profile of abundant membrane lipids that are characteristic of cell

    type. Significantly, the spatial control in both deposition and extraction steps

    combined with the sensitivity of the mass spectrometric detection allows for

    robust molecular profiling of individual cells.

Publication Date


  • 2012

Citation


  • Ellis, S. R., Ferris, C. J., Gilmore, K. J., Mitchell, T. W., Blanksby, S. J. & in het Panhuis, M. (2012). Direct lipid profiling of single cells from inkjet printed microarrays. Analytical Chemistry, 84 (22), 9679-9683.

Scopus Eid


  • 2-s2.0-84869384103

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/4633

Number Of Pages


  • 4

Start Page


  • 9679

End Page


  • 9683

Volume


  • 84

Issue


  • 22

Abstract


  • The on-demand printing of living cells using inkjet

    technologies has recently been demonstrated and allows for the controlled

    deposition of cells in microarrays. Here, we show that such arrays can be

    interrogated directly by robot-controlled liquid microextraction coupled with

    chip-based nanoelectospray mass spectrometry. Such automated analyses

    generate a profile of abundant membrane lipids that are characteristic of cell

    type. Significantly, the spatial control in both deposition and extraction steps

    combined with the sensitivity of the mass spectrometric detection allows for

    robust molecular profiling of individual cells.

Publication Date


  • 2012

Citation


  • Ellis, S. R., Ferris, C. J., Gilmore, K. J., Mitchell, T. W., Blanksby, S. J. & in het Panhuis, M. (2012). Direct lipid profiling of single cells from inkjet printed microarrays. Analytical Chemistry, 84 (22), 9679-9683.

Scopus Eid


  • 2-s2.0-84869384103

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/4633

Number Of Pages


  • 4

Start Page


  • 9679

End Page


  • 9683

Volume


  • 84

Issue


  • 22