Skip to main content

Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells

Journal Article


Download full-text (Open Access)

Abstract


  • Epithelial cells are important in inflammation and immunity. In this study, we examined if Madin–Darby canine kidney (MDCK) epithelial cells express functional P2X7 receptors, which bind the damage-associated molecular pattern extracellular adenosine 5′-triphosphate (ATP). Reverse transcription (RT)-PCR and immunoblotting revealed the expression of P2X7 in MDCK cells. A flow cytometric assay demonstrated that ATP or 2′(3′)-O-(4-benzoylbenzoyl)ATP induced ethidium+ uptake into MDCK cells, and that this process was impaired by the P2X7 antagonists KN-62 and A438079. RT-PCR also demonstrated the presence of Toll-like receptor 4, NALP3, caspase-1, interleukin-1β and interleukin-18 in MDCK cells, as well as in positive control LPS-primed canine monocytes. In conclusion, the MDCK epithelial cell line expresses functional P2X7, as well as Toll-like receptor 4 and molecules associated with the NALP3 inflammasome. This cell line may help elucidate the role of these molecules in kidney epithelial cells and renal disorders in dogs and humans.

Authors


  •   Jalilian, Iman (external author)
  •   Spildrejorde, Mari (external author)
  •   Seavers, Aine (external author)
  •   Curtis, Belinda L. (external author)
  •   McArthur, Jason D.
  •   Sluyter, Ronald

Publication Date


  • 2012

Citation


  • Jalilian, I., Spildrejorde, M., Seavers, A., Curtis, B. L., McArthur, J. D. & Sluyter, R. (2012). Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells. Veterinary Immunology and Immunopathology, 150 (3-4), 228-233.

Scopus Eid


  • 2-s2.0-84868458461

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1206&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/191

Number Of Pages


  • 5

Start Page


  • 228

End Page


  • 233

Volume


  • 150

Issue


  • 3-4

Abstract


  • Epithelial cells are important in inflammation and immunity. In this study, we examined if Madin–Darby canine kidney (MDCK) epithelial cells express functional P2X7 receptors, which bind the damage-associated molecular pattern extracellular adenosine 5′-triphosphate (ATP). Reverse transcription (RT)-PCR and immunoblotting revealed the expression of P2X7 in MDCK cells. A flow cytometric assay demonstrated that ATP or 2′(3′)-O-(4-benzoylbenzoyl)ATP induced ethidium+ uptake into MDCK cells, and that this process was impaired by the P2X7 antagonists KN-62 and A438079. RT-PCR also demonstrated the presence of Toll-like receptor 4, NALP3, caspase-1, interleukin-1β and interleukin-18 in MDCK cells, as well as in positive control LPS-primed canine monocytes. In conclusion, the MDCK epithelial cell line expresses functional P2X7, as well as Toll-like receptor 4 and molecules associated with the NALP3 inflammasome. This cell line may help elucidate the role of these molecules in kidney epithelial cells and renal disorders in dogs and humans.

Authors


  •   Jalilian, Iman (external author)
  •   Spildrejorde, Mari (external author)
  •   Seavers, Aine (external author)
  •   Curtis, Belinda L. (external author)
  •   McArthur, Jason D.
  •   Sluyter, Ronald

Publication Date


  • 2012

Citation


  • Jalilian, I., Spildrejorde, M., Seavers, A., Curtis, B. L., McArthur, J. D. & Sluyter, R. (2012). Functional expression of the damage-associated molecular pattern receptor P2X7 on canine kidney epithelial cells. Veterinary Immunology and Immunopathology, 150 (3-4), 228-233.

Scopus Eid


  • 2-s2.0-84868458461

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1206&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/191

Number Of Pages


  • 5

Start Page


  • 228

End Page


  • 233

Volume


  • 150

Issue


  • 3-4