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Chemical composition and biological activities of the essential oil from leaves of Cleidion javanicum Bl

Journal Article


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Abstract


  • The essential oil from the leaves of Cleidion javanicum Bl. was isolated by hydrodistillation with the percentage yield of 0.003 % as a pale yellow liquid. The composition of the essential oil was analysed by means of GC-(FID) and GC-MS. Ten constituents accounting for 92.60% total oil were identified. The major components were ethyl linoleolate (32.12 %), hexadecanoic acid (26.77 %), trans-phytol (24.64 %) and iso-phytol (4.80 %). The antimicrobial, anticancer, antioxidant activities and cytotoxicity test of this essential oil were investigated. The oil showed non-cytotoxic effects against Vero cells (African green monky kidney) because it inhibited more than 50 % cell growth. The anticancer activity of the essential oil was performed using the Resazurin Microplate Assay (REMA). The oil showed anticancer activity against three cancerous human cell lines; KB-Oral Cavity Cancer, MCF7-Breast Cancer and NCI-H187-Small Cell Lung Cancer with the IC50 of 47.16 μgmL-1, 40.23 μgmL-1 and 49.95 μgmL-1 respectively. The oil also showed antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with the Minimum Inhibition Concentration (MIC) values of 25.00 mg/mL and 12.50 mg/mL respectively, using agar diffusion method. In addition, the essential oil also showed significant antioxidant activity with the IC50 of 27.05 mg mL-1 by means of DPPH radical scavenging assay.

UOW Authors


  •   Sanseera, Duangsuree (external author)
  •   Niwatananun, Wirat (external author)
  •   Liawruangrath, Boonsom (external author)
  •   Liawruangrath, Saisunee (external author)
  •   Baramee, Apiwat (external author)
  •   Pyne, Stephen

Publication Date


  • 2012

Citation


  • Sanseera, D., Niwatananun, W., Liawruangrath, B., Liawruangrath, S., Baramee, A. & Pyne, S. G. (2012). Chemical composition and biological activities of the essential oil from leaves of Cleidion javanicum Bl. Journal of Essential Oil-Bearing Plants, 15 (2), 186-194.

Scopus Eid


  • 2-s2.0-84862259943

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1181&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/182

Number Of Pages


  • 8

Start Page


  • 186

End Page


  • 194

Volume


  • 15

Issue


  • 2

Abstract


  • The essential oil from the leaves of Cleidion javanicum Bl. was isolated by hydrodistillation with the percentage yield of 0.003 % as a pale yellow liquid. The composition of the essential oil was analysed by means of GC-(FID) and GC-MS. Ten constituents accounting for 92.60% total oil were identified. The major components were ethyl linoleolate (32.12 %), hexadecanoic acid (26.77 %), trans-phytol (24.64 %) and iso-phytol (4.80 %). The antimicrobial, anticancer, antioxidant activities and cytotoxicity test of this essential oil were investigated. The oil showed non-cytotoxic effects against Vero cells (African green monky kidney) because it inhibited more than 50 % cell growth. The anticancer activity of the essential oil was performed using the Resazurin Microplate Assay (REMA). The oil showed anticancer activity against three cancerous human cell lines; KB-Oral Cavity Cancer, MCF7-Breast Cancer and NCI-H187-Small Cell Lung Cancer with the IC50 of 47.16 μgmL-1, 40.23 μgmL-1 and 49.95 μgmL-1 respectively. The oil also showed antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with the Minimum Inhibition Concentration (MIC) values of 25.00 mg/mL and 12.50 mg/mL respectively, using agar diffusion method. In addition, the essential oil also showed significant antioxidant activity with the IC50 of 27.05 mg mL-1 by means of DPPH radical scavenging assay.

UOW Authors


  •   Sanseera, Duangsuree (external author)
  •   Niwatananun, Wirat (external author)
  •   Liawruangrath, Boonsom (external author)
  •   Liawruangrath, Saisunee (external author)
  •   Baramee, Apiwat (external author)
  •   Pyne, Stephen

Publication Date


  • 2012

Citation


  • Sanseera, D., Niwatananun, W., Liawruangrath, B., Liawruangrath, S., Baramee, A. & Pyne, S. G. (2012). Chemical composition and biological activities of the essential oil from leaves of Cleidion javanicum Bl. Journal of Essential Oil-Bearing Plants, 15 (2), 186-194.

Scopus Eid


  • 2-s2.0-84862259943

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1181&context=smhpapers

Ro Metadata Url


  • http://ro.uow.edu.au/smhpapers/182

Number Of Pages


  • 8

Start Page


  • 186

End Page


  • 194

Volume


  • 15

Issue


  • 2