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Phospholipid peroxidation: lack of effect of fatty acid pairing

Journal Article


Abstract


  • Phospholipids where both fatty acids are polyunsaturated are very rare. Most organisms prefer to couple their polyunsaturated fatty acids (PUFA) with either a saturated (SAT) or a monounsaturated (MUFA) fatty acid. This study examined if these natural couplings are there to protect PUFA from themselves. Specifically, does the coupling of PUFA to SAT or MUFA reduce the potential for increased rates of peroxidation by shrouding these highly peroxidisable fatty acids with less peroxidisable fatty acids? The influence of head group was examined by using the two most common phospholipids found in vertebrate membranes i.e. phosphatidylcholine and phosphatidylethanolamine species. Fatty acid pairings included 16:0/18:2 versus 18:2/18:2 and 16:0/22:6 versus 22:6/22:6. All phospholipids were incorporated into liposomes that were matched for their total PUFA content i.e. 25% PUFA/PUFA or 50% SAT/PUFA with phosphatidylcholine 16:0/16:0 used as the background phospholipid. An iron initiator (Fe2+/H2O2) was used to induce peroxidation and lipid hydroperoxide production was used to measure peroxidation. The results show that coupling of PUFA together on the same molecule does not increase peroxidation rates and therefore does not support the proposed hypothesis. The lower than expected levels of peroxidation measured for some phospholipid species (e.g. PtdEtn 22:6/22:6) is possibly due to the partitioning of these molecular species into the inner leaflet of the bilayer.

Publication Date


  • 2012

Published In


Citation


  • Norris, S. E., Mitchell, T. W. & Else, P. L. (2012). Phospholipid peroxidation: lack of effect of fatty acid pairing. Lipids, 47 (5), 451-460.

Scopus Eid


  • 2-s2.0-84863498725

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/188

Number Of Pages


  • 9

Start Page


  • 451

End Page


  • 460

Volume


  • 47

Issue


  • 5

Place Of Publication


  • http://download.springer.com/static/pdf/232/art%253A10.1007%252Fs11745-012-3654-5.pdf?auth66=1352695878_bbb67a2e8b588c22895d67b0e4bd048e&ext=.pdf

Abstract


  • Phospholipids where both fatty acids are polyunsaturated are very rare. Most organisms prefer to couple their polyunsaturated fatty acids (PUFA) with either a saturated (SAT) or a monounsaturated (MUFA) fatty acid. This study examined if these natural couplings are there to protect PUFA from themselves. Specifically, does the coupling of PUFA to SAT or MUFA reduce the potential for increased rates of peroxidation by shrouding these highly peroxidisable fatty acids with less peroxidisable fatty acids? The influence of head group was examined by using the two most common phospholipids found in vertebrate membranes i.e. phosphatidylcholine and phosphatidylethanolamine species. Fatty acid pairings included 16:0/18:2 versus 18:2/18:2 and 16:0/22:6 versus 22:6/22:6. All phospholipids were incorporated into liposomes that were matched for their total PUFA content i.e. 25% PUFA/PUFA or 50% SAT/PUFA with phosphatidylcholine 16:0/16:0 used as the background phospholipid. An iron initiator (Fe2+/H2O2) was used to induce peroxidation and lipid hydroperoxide production was used to measure peroxidation. The results show that coupling of PUFA together on the same molecule does not increase peroxidation rates and therefore does not support the proposed hypothesis. The lower than expected levels of peroxidation measured for some phospholipid species (e.g. PtdEtn 22:6/22:6) is possibly due to the partitioning of these molecular species into the inner leaflet of the bilayer.

Publication Date


  • 2012

Published In


Citation


  • Norris, S. E., Mitchell, T. W. & Else, P. L. (2012). Phospholipid peroxidation: lack of effect of fatty acid pairing. Lipids, 47 (5), 451-460.

Scopus Eid


  • 2-s2.0-84863498725

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/188

Number Of Pages


  • 9

Start Page


  • 451

End Page


  • 460

Volume


  • 47

Issue


  • 5

Place Of Publication


  • http://download.springer.com/static/pdf/232/art%253A10.1007%252Fs11745-012-3654-5.pdf?auth66=1352695878_bbb67a2e8b588c22895d67b0e4bd048e&ext=.pdf