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Analysis of unsaturated lipids by ozone-induced dissociation

Journal Article


Abstract


  • Recent developments in analytical technologies have driven significant advances in lipid science. The sensitivity and selectivity of modern mass spectrometers can now provide for the detection and even quantification of many hundreds of lipids in a single analysis. In parallel, increasing evidence from structural biology suggests that a detailed knowledge of lipid molecular structure including carbon-carbon double bond position, stereochemistry and acyl chain regiochemistry is required to fully appreciate the biochemical role(s) of individual lipids. Here we review the capabilities and limitations of tandem mass spectrometry to provide this level of structural specificity in the analysis of lipids present in complex biological extracts. In particular, we focus on the capabilities of a novel technology termed ozone-induced dissociation to identify the position(s) of double bonds in unsaturated lipids and discuss its possible role in efforts to develop workflows that provide for complete structure elucidation of lipids by mass spectrometry alone: so-called top-down lipidomics. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrom. © 2011 Elsevier B.V

UOW Authors


  •   Brown, Simon (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2011

Citation


  • Brown, S. H.J., Mitchell, T. W. & Blanksby, S. J. (2011). Analysis of unsaturated lipids by ozone-induced dissociation. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 1811 (11), 807-817.

Scopus Eid


  • 2-s2.0-80255123435

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5296

Number Of Pages


  • 10

Start Page


  • 807

End Page


  • 817

Volume


  • 1811

Issue


  • 11

Abstract


  • Recent developments in analytical technologies have driven significant advances in lipid science. The sensitivity and selectivity of modern mass spectrometers can now provide for the detection and even quantification of many hundreds of lipids in a single analysis. In parallel, increasing evidence from structural biology suggests that a detailed knowledge of lipid molecular structure including carbon-carbon double bond position, stereochemistry and acyl chain regiochemistry is required to fully appreciate the biochemical role(s) of individual lipids. Here we review the capabilities and limitations of tandem mass spectrometry to provide this level of structural specificity in the analysis of lipids present in complex biological extracts. In particular, we focus on the capabilities of a novel technology termed ozone-induced dissociation to identify the position(s) of double bonds in unsaturated lipids and discuss its possible role in efforts to develop workflows that provide for complete structure elucidation of lipids by mass spectrometry alone: so-called top-down lipidomics. This article is part of a Special Issue entitled: Lipodomics and Imaging Mass Spectrom. © 2011 Elsevier B.V

UOW Authors


  •   Brown, Simon (external author)
  •   Mitchell, Todd
  •   Blanksby, Stephen J. (external author)

Publication Date


  • 2011

Citation


  • Brown, S. H.J., Mitchell, T. W. & Blanksby, S. J. (2011). Analysis of unsaturated lipids by ozone-induced dissociation. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 1811 (11), 807-817.

Scopus Eid


  • 2-s2.0-80255123435

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5296

Number Of Pages


  • 10

Start Page


  • 807

End Page


  • 817

Volume


  • 1811

Issue


  • 11