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Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry

Journal Article


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Abstract


  • Meibum is believed to be the major source of tear !lm lipids, which are vital in the prevention of excess

    evaporation of the aqueous phase. The complete lipid composition of meibum has yet to be established.

    While earlier studies reported the presence of phospholipids in human meibum, recent mass spectrometric

    studies have not detected them. In this study we use electrospray ionisation tandem mass

    spectrometry to investigate the presence of phospholipids in meibum and provide comparison to the

    phospholipid pro!le of tears.

    Lipids were extracted from human meibum and tear samples using standard biphasic methods and

    analysed by nano-electrospray ionisation tandem mass spectrometry using targeted ion scans. A total of

    35 choline-containing phospholipids were identi!ed in meibum and the pro!le of these was similar to

    that observed in tears, suggesting tear lipids are derived from meibum. The results shown here highlight

    the need for a combination of optimised techniques to enable the identi!cation of the large range of lipid

    classes in meibum.

UOW Authors


  •   Saville, Jennifer T. (external author)
  •   Zhao, Zhenjun (external author)
  •   Willcox, Mark D. P. (external author)
  •   Ariyavidana, Manjula A. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2011

Citation


  • Saville, J. T., Zhao, Z., Willcox, M. D. P., Ariyavidana, M. A., Blanksby, S. J. & Mitchell, T. W. (2011). Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry. Experimental Eye Research, 92 (3), 238-240.

Scopus Eid


  • 2-s2.0-79952101281

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6395&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/3053

Has Global Citation Frequency


Number Of Pages


  • 2

Start Page


  • 238

End Page


  • 240

Volume


  • 92

Issue


  • 3

Abstract


  • Meibum is believed to be the major source of tear !lm lipids, which are vital in the prevention of excess

    evaporation of the aqueous phase. The complete lipid composition of meibum has yet to be established.

    While earlier studies reported the presence of phospholipids in human meibum, recent mass spectrometric

    studies have not detected them. In this study we use electrospray ionisation tandem mass

    spectrometry to investigate the presence of phospholipids in meibum and provide comparison to the

    phospholipid pro!le of tears.

    Lipids were extracted from human meibum and tear samples using standard biphasic methods and

    analysed by nano-electrospray ionisation tandem mass spectrometry using targeted ion scans. A total of

    35 choline-containing phospholipids were identi!ed in meibum and the pro!le of these was similar to

    that observed in tears, suggesting tear lipids are derived from meibum. The results shown here highlight

    the need for a combination of optimised techniques to enable the identi!cation of the large range of lipid

    classes in meibum.

UOW Authors


  •   Saville, Jennifer T. (external author)
  •   Zhao, Zhenjun (external author)
  •   Willcox, Mark D. P. (external author)
  •   Ariyavidana, Manjula A. (external author)
  •   Blanksby, Stephen J. (external author)
  •   Mitchell, Todd

Publication Date


  • 2011

Citation


  • Saville, J. T., Zhao, Z., Willcox, M. D. P., Ariyavidana, M. A., Blanksby, S. J. & Mitchell, T. W. (2011). Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry. Experimental Eye Research, 92 (3), 238-240.

Scopus Eid


  • 2-s2.0-79952101281

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=6395&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/3053

Has Global Citation Frequency


Number Of Pages


  • 2

Start Page


  • 238

End Page


  • 240

Volume


  • 92

Issue


  • 3