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Kinase inhibitory, haemolytic and cytotoxic activity of three deep-water sponges from North Western Australia and their fatty acid composition

Journal Article


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Abstract


  • The c-AMP dependent protein kinase (PKA) inhibition, haemolytic activity, and cytoxicity of 21 extracts obtained from North Western Australian sponges collected from depths of 84-135 m were investigated. Hexane extracts from Ircinia/Sarcotragus sp. and Geodia sp. displayed PKA inhibitory activities of 100 and 97% respectively (at 100 μg/mL), while aq. methanol extracts from Haliclona sp. exhibited potent haemolytic activity (75%) and hexane extracts from Geodia sp. were highly toxic (88%) to the brine shrimp Artemia franciscana. As the non-polar extracts gave the greatest PKA inhibition, these were further analysed by GC-MS and 29 fatty acids were identified in the highest proportions in Ircinia/Sarcotragus sp. > Haliclona sp. > Geodia sp. In contrast to shallow-water sponges that are dominated by polyunsaturated fatty acids with a high percentage of long chain fatty acids, LCFAs (C24-C30), the deep-sea sponges investigated herein were all found to be rich in saturated fatty acids, in particular C14-C20 fatty acids, including odd and branched chain fatty acids, with only low levels (0-10%) of LCFAs. Screening of the PKA inhibitory activity of a series of commercially available fatty acids identified C14-C18 fatty acids as possessing significant PKA inhibitory activity that may contribute to the activity observed in the sponges studied.

Authors


  •   Zivanovic, Ana (external author)
  •   Pastro, Natalie J. (external author)
  •   Fromont, Jane (external author)
  •   Thomson, Murray (external author)
  •   Skropeta, Danielle

Publication Date


  • 2011

Citation


  • Zivanovic, A., Pastro, N. J., Fromont, J., Thomson, M. & Skropeta, D. (2011). Kinase inhibitory, haemolytic and cytotoxic activity of three deep-water sponges from North Western Australia and their fatty acid composition. Natural Product Communications: an international journal for communications and reviews, 6 (12), 1921-1924.

Scopus Eid


  • 2-s2.0-80054952156

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=8629&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5286

Number Of Pages


  • 3

Start Page


  • 1921

End Page


  • 1924

Volume


  • 6

Issue


  • 12

Place Of Publication


  • http://www.naturalproduct.us/

Abstract


  • The c-AMP dependent protein kinase (PKA) inhibition, haemolytic activity, and cytoxicity of 21 extracts obtained from North Western Australian sponges collected from depths of 84-135 m were investigated. Hexane extracts from Ircinia/Sarcotragus sp. and Geodia sp. displayed PKA inhibitory activities of 100 and 97% respectively (at 100 μg/mL), while aq. methanol extracts from Haliclona sp. exhibited potent haemolytic activity (75%) and hexane extracts from Geodia sp. were highly toxic (88%) to the brine shrimp Artemia franciscana. As the non-polar extracts gave the greatest PKA inhibition, these were further analysed by GC-MS and 29 fatty acids were identified in the highest proportions in Ircinia/Sarcotragus sp. > Haliclona sp. > Geodia sp. In contrast to shallow-water sponges that are dominated by polyunsaturated fatty acids with a high percentage of long chain fatty acids, LCFAs (C24-C30), the deep-sea sponges investigated herein were all found to be rich in saturated fatty acids, in particular C14-C20 fatty acids, including odd and branched chain fatty acids, with only low levels (0-10%) of LCFAs. Screening of the PKA inhibitory activity of a series of commercially available fatty acids identified C14-C18 fatty acids as possessing significant PKA inhibitory activity that may contribute to the activity observed in the sponges studied.

Authors


  •   Zivanovic, Ana (external author)
  •   Pastro, Natalie J. (external author)
  •   Fromont, Jane (external author)
  •   Thomson, Murray (external author)
  •   Skropeta, Danielle

Publication Date


  • 2011

Citation


  • Zivanovic, A., Pastro, N. J., Fromont, J., Thomson, M. & Skropeta, D. (2011). Kinase inhibitory, haemolytic and cytotoxic activity of three deep-water sponges from North Western Australia and their fatty acid composition. Natural Product Communications: an international journal for communications and reviews, 6 (12), 1921-1924.

Scopus Eid


  • 2-s2.0-80054952156

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=8629&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5286

Number Of Pages


  • 3

Start Page


  • 1921

End Page


  • 1924

Volume


  • 6

Issue


  • 12

Place Of Publication


  • http://www.naturalproduct.us/