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Regulation of alpha-synuclein expression by liver X receptor ligands in vitro

Journal Article


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Abstract


  • Alpha-synuclein is a lipid-binding protein expressed in neurons and oligodendrocytes which is increased in Parkinson's disease. We identified two putative liver X receptor (LXR) response elements in the human oc-synuclein gene and used synthetic (TO901317, GW3695) and physiological (27-hydroxycholesterol) LXR activators to assess regulation of α-synuclein. LXR ligands upregulated α-synuclein m RNA by two-five-fold in human SK-N-SH neurons and three-six-fold in human MO3.13 oligodendrocytes. Significant 50% to four-fold induction of α-synuclein protein was also detected. Under these conditions, m RNA for LXR-responsive gene ABCAI was significantly upregulated 15-40-fold and 5-25-fold in neurons and oligodendrocytes, respectively. LXR may, therefore, contribute to the regulation of α-synuclein expression in neurons and oligodendrocytes. © 2008 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Authors


  •   Cheng, Danni (external author)
  •   Kim, Woojin S. (external author)
  •   Garner, Brett

Publication Date


  • 2008

Citation


  • Cheng, D., Kim, W. & Garner, B. (2008). Regulation of alpha-synuclein expression by liver X receptor ligands in vitro. NeuroReport, 19 (17), 1685-1689.

Scopus Eid


  • 2-s2.0-56749091143

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1038&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/23

Number Of Pages


  • 4

Start Page


  • 1685

End Page


  • 1689

Volume


  • 19

Issue


  • 17

Abstract


  • Alpha-synuclein is a lipid-binding protein expressed in neurons and oligodendrocytes which is increased in Parkinson's disease. We identified two putative liver X receptor (LXR) response elements in the human oc-synuclein gene and used synthetic (TO901317, GW3695) and physiological (27-hydroxycholesterol) LXR activators to assess regulation of α-synuclein. LXR ligands upregulated α-synuclein m RNA by two-five-fold in human SK-N-SH neurons and three-six-fold in human MO3.13 oligodendrocytes. Significant 50% to four-fold induction of α-synuclein protein was also detected. Under these conditions, m RNA for LXR-responsive gene ABCAI was significantly upregulated 15-40-fold and 5-25-fold in neurons and oligodendrocytes, respectively. LXR may, therefore, contribute to the regulation of α-synuclein expression in neurons and oligodendrocytes. © 2008 Wolters Kluwer Health | Lippincott Williams & Wilkins.

Authors


  •   Cheng, Danni (external author)
  •   Kim, Woojin S. (external author)
  •   Garner, Brett

Publication Date


  • 2008

Citation


  • Cheng, D., Kim, W. & Garner, B. (2008). Regulation of alpha-synuclein expression by liver X receptor ligands in vitro. NeuroReport, 19 (17), 1685-1689.

Scopus Eid


  • 2-s2.0-56749091143

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1038&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/23

Number Of Pages


  • 4

Start Page


  • 1685

End Page


  • 1689

Volume


  • 19

Issue


  • 17