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Fluorescence and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells

Journal Article


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Abstract


  • Frequency domain fluorescence lifetime imaging microscopy (FLIM) has been used in combination with laser scanning confocal microscopy to study the cellular uptake behavior of the antitumor drug doxorubicin (DOX) and micellar-encapsulated DOX (PLyAd-DOX). The endocytosis uptake process of PLyAd-DOX was monitored over 72 hours using confocal microscopy, with a maximum fluorescence recorded at incubation periods around 24 hours. The micellar structure was not found to release the encapsulated DOX during the time course of imaging. FLIM revealed single lifetime distributions of PLyAd-DOX during accumulation in the cytoplasm. The free DOX in contrast was observed both in the cytoplasm and the nuclear domain of the cell, showing bimodal lifetime distributions. There was a marked dependence of the measured free-DOX lifetime on concentration within the cell, in contrast to reference experiments in aqueous solution, where no such dependence was found. The results suggest the formation of macromolecular structures inside the living cells. © 2008 Elsevier Inc. All rights reserved.

UOW Authors


  •   Dai, X F. (external author)
  •   Yue, Zhilian
  •   Eccleston, M E (external author)
  •   Swartling, J (external author)
  •   Slater, Nigel K. H. (external author)
  •   Kaminski, C F (external author)

Publication Date


  • 2008

Citation


  • Dai, X., yue, z., Eccleston, M., Swartling, J., Slater, N. & Kaminski, C. (2008). Fluorescence and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells. Nanomedicine: Nanotechnology, Biology and Medicine, 4 (1), 49-56.

Scopus Eid


  • 2-s2.0-40049086555

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1919&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/880

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 49

End Page


  • 56

Volume


  • 4

Issue


  • 1

Abstract


  • Frequency domain fluorescence lifetime imaging microscopy (FLIM) has been used in combination with laser scanning confocal microscopy to study the cellular uptake behavior of the antitumor drug doxorubicin (DOX) and micellar-encapsulated DOX (PLyAd-DOX). The endocytosis uptake process of PLyAd-DOX was monitored over 72 hours using confocal microscopy, with a maximum fluorescence recorded at incubation periods around 24 hours. The micellar structure was not found to release the encapsulated DOX during the time course of imaging. FLIM revealed single lifetime distributions of PLyAd-DOX during accumulation in the cytoplasm. The free DOX in contrast was observed both in the cytoplasm and the nuclear domain of the cell, showing bimodal lifetime distributions. There was a marked dependence of the measured free-DOX lifetime on concentration within the cell, in contrast to reference experiments in aqueous solution, where no such dependence was found. The results suggest the formation of macromolecular structures inside the living cells. © 2008 Elsevier Inc. All rights reserved.

UOW Authors


  •   Dai, X F. (external author)
  •   Yue, Zhilian
  •   Eccleston, M E (external author)
  •   Swartling, J (external author)
  •   Slater, Nigel K. H. (external author)
  •   Kaminski, C F (external author)

Publication Date


  • 2008

Citation


  • Dai, X., yue, z., Eccleston, M., Swartling, J., Slater, N. & Kaminski, C. (2008). Fluorescence and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells. Nanomedicine: Nanotechnology, Biology and Medicine, 4 (1), 49-56.

Scopus Eid


  • 2-s2.0-40049086555

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1919&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/880

Has Global Citation Frequency


Number Of Pages


  • 7

Start Page


  • 49

End Page


  • 56

Volume


  • 4

Issue


  • 1