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Molecular characterization of the onset and progression of colitis in inoculated interleukin-10 gene-deficient mice: a role for PPARcx

Journal Article


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Abstract


  • The interleukin-10 gene-deficient (Il10−/−) mouse is a model of human inflammatory bowel disease and Ppara has been identified

    as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10−/− model. The aims were to (1) characterize

    colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between

    PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10−/− mice from 10 to 12 weeks

    of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton

    dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in

    12-week-old Il10−/− mice. Gene expression changes in 12-week-old Il10−/− mice were related to PPARα signaling likely to control

    colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   Cooney, Janine (external author)
  •   McNabb, Warren C. (external author)
  •   Barraclough, Diane (external author)
  •   Laing, William (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   MacLean, Paul (external author)
  •   Knowles, Scott O. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2010

Citation


  • Knock, B., Barnett, M. P. G., Cooney, J., McNabb, W. C., Barraclough, D., Laing, W., Zhu, S., Park, Z. A., MacLean, P., Knowles, S. O. & Roy, N. (2010). Molecular characterization of the onset and progression of colitis in inoculated interleukin-10 gene-deficient mice: a role for PPARcx. PPAR Research, 2010 (Article ID 621069), 1-18.

Scopus Eid


  • 2-s2.0-77955369664

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1025&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/11

Has Global Citation Frequency


Number Of Pages


  • 17

Start Page


  • 1

End Page


  • 18

Volume


  • 2010

Issue


  • Article ID 621069

Abstract


  • The interleukin-10 gene-deficient (Il10−/−) mouse is a model of human inflammatory bowel disease and Ppara has been identified

    as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10−/− model. The aims were to (1) characterize

    colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between

    PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10−/− mice from 10 to 12 weeks

    of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton

    dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in

    12-week-old Il10−/− mice. Gene expression changes in 12-week-old Il10−/− mice were related to PPARα signaling likely to control

    colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   Cooney, Janine (external author)
  •   McNabb, Warren C. (external author)
  •   Barraclough, Diane (external author)
  •   Laing, William (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   MacLean, Paul (external author)
  •   Knowles, Scott O. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2010

Citation


  • Knock, B., Barnett, M. P. G., Cooney, J., McNabb, W. C., Barraclough, D., Laing, W., Zhu, S., Park, Z. A., MacLean, P., Knowles, S. O. & Roy, N. (2010). Molecular characterization of the onset and progression of colitis in inoculated interleukin-10 gene-deficient mice: a role for PPARcx. PPAR Research, 2010 (Article ID 621069), 1-18.

Scopus Eid


  • 2-s2.0-77955369664

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1025&context=ihmri

Ro Metadata Url


  • http://ro.uow.edu.au/ihmri/11

Has Global Citation Frequency


Number Of Pages


  • 17

Start Page


  • 1

End Page


  • 18

Volume


  • 2010

Issue


  • Article ID 621069