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Dietary arachidonic acid-mediated effects on colon inflammation using transcriptome analysis

Journal Article


Abstract


  • Increased levels of n-6 arachidonic acid (AA), a precursor of pro-inflammatory eicosanoids, have

    been found in the colon mucosa of inflammatory bowel disease patients when compared with

    healthy subjects. The hypothesis was that dietary AA would aggravate colon inflammation by

    changing expression of genes in inflammatory signaling pathways. AA-enriched diet was fed to

    IL10 gene-deficient (Il10/) mice, model of a inflammatory bowel disease, and compared with

    Il10/ mice fed an oleic acid control diet. Effects of AA on gene expression profiles during

    colitis were examined using whole genome microarray analysis. Dietary AA decreased the

    expression levels of some colonic genes in ER stress, complement system, nuclear respiratory

    factor 2-mediated oxidative stress and positive acute phase response pathways compared with

    Il10/ mice fed an oleic acid diet. AA increased the expression levels of fatty acid catabolism

    genes, but decreased that of lipid synthesis genes during colitis, likely by sterol regulatory

    element binding transcription factor 1 and target gene regulation. A link has been suggested

    between AA and reduction of intestinal fibrosis by down-regulating the expression levels of proinflammatory

    and fibrotic marker genes. Contrary to the hypothesis, these findings suggest that

    dietary AA, in the present experimental conditions, is not pro-inflammatory, reduces ER stress

    and protects colonocytes from oxidative stress in Il10/ mice.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   McNabb, Warren C. (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   Khan, Anar (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2010

Citation


  • Knock, B., Barnett, M. P. G., McNabb, W. C., Zhu, S., Park, Z. A., Khan, A. & Roy, N. (2010). Dietary arachidonic acid-mediated effects on colon inflammation using transcriptome analysis. Molecular Nutrition & Food Research, 54 (Supp.1), S62-S74.

Scopus Eid


  • 2-s2.0-77952362187

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5203

Has Global Citation Frequency


Start Page


  • S62

End Page


  • S74

Volume


  • 54

Issue


  • Supp.1

Abstract


  • Increased levels of n-6 arachidonic acid (AA), a precursor of pro-inflammatory eicosanoids, have

    been found in the colon mucosa of inflammatory bowel disease patients when compared with

    healthy subjects. The hypothesis was that dietary AA would aggravate colon inflammation by

    changing expression of genes in inflammatory signaling pathways. AA-enriched diet was fed to

    IL10 gene-deficient (Il10/) mice, model of a inflammatory bowel disease, and compared with

    Il10/ mice fed an oleic acid control diet. Effects of AA on gene expression profiles during

    colitis were examined using whole genome microarray analysis. Dietary AA decreased the

    expression levels of some colonic genes in ER stress, complement system, nuclear respiratory

    factor 2-mediated oxidative stress and positive acute phase response pathways compared with

    Il10/ mice fed an oleic acid diet. AA increased the expression levels of fatty acid catabolism

    genes, but decreased that of lipid synthesis genes during colitis, likely by sterol regulatory

    element binding transcription factor 1 and target gene regulation. A link has been suggested

    between AA and reduction of intestinal fibrosis by down-regulating the expression levels of proinflammatory

    and fibrotic marker genes. Contrary to the hypothesis, these findings suggest that

    dietary AA, in the present experimental conditions, is not pro-inflammatory, reduces ER stress

    and protects colonocytes from oxidative stress in Il10/ mice.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   McNabb, Warren C. (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   Khan, Anar (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2010

Citation


  • Knock, B., Barnett, M. P. G., McNabb, W. C., Zhu, S., Park, Z. A., Khan, A. & Roy, N. (2010). Dietary arachidonic acid-mediated effects on colon inflammation using transcriptome analysis. Molecular Nutrition & Food Research, 54 (Supp.1), S62-S74.

Scopus Eid


  • 2-s2.0-77952362187

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/5203

Has Global Citation Frequency


Start Page


  • S62

End Page


  • S74

Volume


  • 54

Issue


  • Supp.1