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Multidrug resistance gene deficient (mdr1a-/-) mice have an altered caecal microbiota that precedes the onset of intestinal inflammation

Journal Article


Abstract


  • Aim: To compare caecal microbiota from mdr1a– ⁄ – and wild type (FVB) mice

    to identify differences in the bacterial community that could influence the

    intestinal inflammation.

    Methods and Results: Caecal microbiota of mdr1a– ⁄ – and FVB mice were evaluated

    at 12 and 25 weeks of age using denaturing gradient gel electrophoresis

    (DGGE) and quantitative real-time PCR. DGGE fingerprints of FVB and

    mdr1a– ⁄ – mice (with no intestinal inflammation) at 12 weeks revealed differences

    in the presence of DNA fragments identified as Bacteroides fragilis,

    B. thetaiotaomicron, B. vulgatus and an uncultured alphaproteobacterium.

    Escherichia coli and Acinetobacter sp. were only identified in DGGE profiles of

    mdr1a– ⁄ – mice at 25 weeks (with severe intestinal inflammation), which also

    had a lower number of total bacteria in the caecum compared with FVB mice

    at same age.

    Conclusions: Differences found in the caecal microbiota of FVB and mdr1a– ⁄ –

    mice (12 weeks) suggest that the lack of Abcb1 transporters in intestinal cells

    due to the disruption of the mdr1a gene might lead to changes in the caecal

    microbiota. The altered microbiota along with the genetic defect could contribute

    to the development of intestinal inflammation in mdr1a– ⁄ – mice.

    Significance and Impact of the Study: Differences in caecal microbiota of

    mdr1a– ⁄ – and FVB mice (12 weeks) suggest genotype specific colonization. The

    results provide evidence that Abcb1 transporters may regulate host interactions

    with commensal bacteria. Future work is needed to identify the mechanisms

    involved in this possible cross-talk between the host intestinal cells and

    microbiota.

UOW Authors


  •   Nones, Katie (external author)
  •   Suesse, Bianca
  •   Dommels, Yvonne (external author)
  •   Paturi, G (external author)
  •   Butts, C (external author)
  •   McNabb, Warren C. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2009

Citation


  • Nones, K., Knock, B., Dommels, Y., Paturi, G., Butts, C., McNabb, W. C. & Roy, N. (2009). Multidrug resistance gene deficient (mdr1a-/-) mice have an altered caecal microbiota that precedes the onset of intestinal inflammation. Journal of Applied Microbiology, 107 (2), 557-566.

Scopus Eid


  • 2-s2.0-68049128275

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 557

End Page


  • 566

Volume


  • 107

Issue


  • 2

Abstract


  • Aim: To compare caecal microbiota from mdr1a– ⁄ – and wild type (FVB) mice

    to identify differences in the bacterial community that could influence the

    intestinal inflammation.

    Methods and Results: Caecal microbiota of mdr1a– ⁄ – and FVB mice were evaluated

    at 12 and 25 weeks of age using denaturing gradient gel electrophoresis

    (DGGE) and quantitative real-time PCR. DGGE fingerprints of FVB and

    mdr1a– ⁄ – mice (with no intestinal inflammation) at 12 weeks revealed differences

    in the presence of DNA fragments identified as Bacteroides fragilis,

    B. thetaiotaomicron, B. vulgatus and an uncultured alphaproteobacterium.

    Escherichia coli and Acinetobacter sp. were only identified in DGGE profiles of

    mdr1a– ⁄ – mice at 25 weeks (with severe intestinal inflammation), which also

    had a lower number of total bacteria in the caecum compared with FVB mice

    at same age.

    Conclusions: Differences found in the caecal microbiota of FVB and mdr1a– ⁄ –

    mice (12 weeks) suggest that the lack of Abcb1 transporters in intestinal cells

    due to the disruption of the mdr1a gene might lead to changes in the caecal

    microbiota. The altered microbiota along with the genetic defect could contribute

    to the development of intestinal inflammation in mdr1a– ⁄ – mice.

    Significance and Impact of the Study: Differences in caecal microbiota of

    mdr1a– ⁄ – and FVB mice (12 weeks) suggest genotype specific colonization. The

    results provide evidence that Abcb1 transporters may regulate host interactions

    with commensal bacteria. Future work is needed to identify the mechanisms

    involved in this possible cross-talk between the host intestinal cells and

    microbiota.

UOW Authors


  •   Nones, Katie (external author)
  •   Suesse, Bianca
  •   Dommels, Yvonne (external author)
  •   Paturi, G (external author)
  •   Butts, C (external author)
  •   McNabb, Warren C. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2009

Citation


  • Nones, K., Knock, B., Dommels, Y., Paturi, G., Butts, C., McNabb, W. C. & Roy, N. (2009). Multidrug resistance gene deficient (mdr1a-/-) mice have an altered caecal microbiota that precedes the onset of intestinal inflammation. Journal of Applied Microbiology, 107 (2), 557-566.

Scopus Eid


  • 2-s2.0-68049128275

Has Global Citation Frequency


Number Of Pages


  • 9

Start Page


  • 557

End Page


  • 566

Volume


  • 107

Issue


  • 2