Background/Aims: Dietary n–3 polyunsaturated fatty acids
can reduce inflammation via a range of mechanisms. This
study tested the effect of dietary eicosapentaenoic acid
(EPA) on intestinal inflammation using interleukin-10 genedeficient
(Il10 –/– ) mice. Methods: At 35 days of age, 12 weaned
Il10 –/– and 12 C57 mice were randomly assigned to one of
two modified AIN-76A diets, supplemented with 3.7% purified
ethyl esters of either EPA (n–3) or oleic acid (OA, control).
To identify genes relevant to colon inflammation, transcription
profiling (microarrays and qRT-PCR) and bioinformatic
analyses were used. Results: In this study, dietary EPA reversed
the decrease in colon fatty acid -oxidation gene expression
observed in OA-fed Il10 –/– compared to C57 mice.
Il10 –/– mice fed the OA diet showed decreased expression of
antioxidant enzyme genes, as well as those involved in detoxification
of xenobiotics, compared to C57 mice on the same diet. In contrast, dietary EPA increased the expression
of these genes in Il10 –/– mice. Conclusions: These data indicate
that dietary EPA-induced endogenous lipid oxidation
which might have a potential anti-inflammatory effect on
colon tissue. This is supported by the activation of the Ppara
gene that regulates the expression of pro-inflammatory and
immunomodulatory genes and proteins.