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Genome-Wide Analysis of Dietary Eicosapentaenoic Acid- and Oleic Acid-Induced Modulation of Colon Inflammation in Interleukin-10 Gene-Deficient Mice

Journal Article


Abstract


  • Background/Aims: Dietary n–3 polyunsaturated fatty acids

    can reduce inflammation via a range of mechanisms. This

    study tested the effect of dietary eicosapentaenoic acid

    (EPA) on intestinal inflammation using interleukin-10 genedeficient

    (Il10 –/– ) mice. Methods: At 35 days of age, 12 weaned

    Il10 –/– and 12 C57 mice were randomly assigned to one of

    two modified AIN-76A diets, supplemented with 3.7% purified

    ethyl esters of either EPA (n–3) or oleic acid (OA, control).

    To identify genes relevant to colon inflammation, transcription

    profiling (microarrays and qRT-PCR) and bioinformatic

    analyses were used. Results: In this study, dietary EPA reversed

    the decrease in colon fatty acid -oxidation gene expression

    observed in OA-fed Il10 –/– compared to C57 mice.

    Il10 –/– mice fed the OA diet showed decreased expression of

    antioxidant enzyme genes, as well as those involved in detoxification

    of xenobiotics, compared to C57 mice on the same diet. In contrast, dietary EPA increased the expression

    of these genes in Il10 –/– mice. Conclusions: These data indicate

    that dietary EPA-induced endogenous lipid oxidation

    which might have a potential anti-inflammatory effect on

    colon tissue. This is supported by the activation of the Ppara

    gene that regulates the expression of pro-inflammatory and

    immunomodulatory genes and proteins.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   Nones, Katie (external author)
  •   Dommels, Yvonne (external author)
  •   Knowles, Scott O. (external author)
  •   McNabb, Warren C. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2009

Citation


  • Knock, B., Barnett, M. P. G., Zhu, S., Park, Z. A., Nones, K., Dommels, Y., Knowles, S. O., McNabb, W. C. & Roy, N. (2009). Genome-Wide Analysis of Dietary Eicosapentaenoic Acid- and Oleic Acid-Induced Modulation of Colon Inflammation in Interleukin-10 Gene-Deficient Mice. Journal of Nutrigenetics and Nutrigenomics, 2 9-28.

Scopus Eid


  • 2-s2.0-61649127766

Has Global Citation Frequency


Number Of Pages


  • 19

Start Page


  • 9

End Page


  • 28

Volume


  • 2

Abstract


  • Background/Aims: Dietary n–3 polyunsaturated fatty acids

    can reduce inflammation via a range of mechanisms. This

    study tested the effect of dietary eicosapentaenoic acid

    (EPA) on intestinal inflammation using interleukin-10 genedeficient

    (Il10 –/– ) mice. Methods: At 35 days of age, 12 weaned

    Il10 –/– and 12 C57 mice were randomly assigned to one of

    two modified AIN-76A diets, supplemented with 3.7% purified

    ethyl esters of either EPA (n–3) or oleic acid (OA, control).

    To identify genes relevant to colon inflammation, transcription

    profiling (microarrays and qRT-PCR) and bioinformatic

    analyses were used. Results: In this study, dietary EPA reversed

    the decrease in colon fatty acid -oxidation gene expression

    observed in OA-fed Il10 –/– compared to C57 mice.

    Il10 –/– mice fed the OA diet showed decreased expression of

    antioxidant enzyme genes, as well as those involved in detoxification

    of xenobiotics, compared to C57 mice on the same diet. In contrast, dietary EPA increased the expression

    of these genes in Il10 –/– mice. Conclusions: These data indicate

    that dietary EPA-induced endogenous lipid oxidation

    which might have a potential anti-inflammatory effect on

    colon tissue. This is supported by the activation of the Ppara

    gene that regulates the expression of pro-inflammatory and

    immunomodulatory genes and proteins.

UOW Authors


  •   Suesse, Bianca
  •   Barnett, Matthew P. G. (external author)
  •   Zhu, Shuotun (external author)
  •   Park, Zaneta A. (external author)
  •   Nones, Katie (external author)
  •   Dommels, Yvonne (external author)
  •   Knowles, Scott O. (external author)
  •   McNabb, Warren C. (external author)
  •   Roy, Nicole C. (external author)

Publication Date


  • 2009

Citation


  • Knock, B., Barnett, M. P. G., Zhu, S., Park, Z. A., Nones, K., Dommels, Y., Knowles, S. O., McNabb, W. C. & Roy, N. (2009). Genome-Wide Analysis of Dietary Eicosapentaenoic Acid- and Oleic Acid-Induced Modulation of Colon Inflammation in Interleukin-10 Gene-Deficient Mice. Journal of Nutrigenetics and Nutrigenomics, 2 9-28.

Scopus Eid


  • 2-s2.0-61649127766

Has Global Citation Frequency


Number Of Pages


  • 19

Start Page


  • 9

End Page


  • 28

Volume


  • 2