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Insulin decreases therapeutic efficacy in colon cancer cell line HT29 via the activation of the PI3K/Akt pathway

Journal Article


Abstract


  • Obesity has been associated with both the carcinogenesis and poor prognosis of colon cancer, one of the leading causes of cancer-related death. Increased blood levels of insulin in obese subjects have been demonstrated to play a key role in carcinogenesis. It is also possible that insulin affects treatment efficacy, leading to poor prognosis. In this study, we demonstrated that insulin can increase HT29 colon cancer cell line resistance to cycloheximide and 5- fluorouracil induced cytotoxicity. This effect can be inhibited by the PI3K/Akt inhibitor Ly294002, indicating the important role of this pathway in the insulin-induced inefficacy of chemotherapy. The insulin-induced resistance to cycloheximide and 5-fluorouracil can be used in drug screening to overcome the inefficacy of chemotherapy in obesity-associated colon cancer.

Authors


  •   Chen, Jiezhong
  •   Katsifis, Andrew (external author)
  •   Hu, Chang-Hua (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2011

Citation


  • Chen, J., Katsifis, A., Hu, C. & Huang, X. (2011). Insulin decreases therapeutic efficacy in colon cancer cell line HT29 via the activation of the PI3K/Akt pathway. Current Drug Discovery Technologies, 8 (2), 119-125.

Scopus Eid


  • 2-s2.0-79956123640

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3553

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 119

End Page


  • 125

Volume


  • 8

Issue


  • 2

Abstract


  • Obesity has been associated with both the carcinogenesis and poor prognosis of colon cancer, one of the leading causes of cancer-related death. Increased blood levels of insulin in obese subjects have been demonstrated to play a key role in carcinogenesis. It is also possible that insulin affects treatment efficacy, leading to poor prognosis. In this study, we demonstrated that insulin can increase HT29 colon cancer cell line resistance to cycloheximide and 5- fluorouracil induced cytotoxicity. This effect can be inhibited by the PI3K/Akt inhibitor Ly294002, indicating the important role of this pathway in the insulin-induced inefficacy of chemotherapy. The insulin-induced resistance to cycloheximide and 5-fluorouracil can be used in drug screening to overcome the inefficacy of chemotherapy in obesity-associated colon cancer.

Authors


  •   Chen, Jiezhong
  •   Katsifis, Andrew (external author)
  •   Hu, Chang-Hua (external author)
  •   Huang, Xu-Feng

Publication Date


  • 2011

Citation


  • Chen, J., Katsifis, A., Hu, C. & Huang, X. (2011). Insulin decreases therapeutic efficacy in colon cancer cell line HT29 via the activation of the PI3K/Akt pathway. Current Drug Discovery Technologies, 8 (2), 119-125.

Scopus Eid


  • 2-s2.0-79956123640

Ro Metadata Url


  • http://ro.uow.edu.au/hbspapers/3553

Has Global Citation Frequency


Number Of Pages


  • 6

Start Page


  • 119

End Page


  • 125

Volume


  • 8

Issue


  • 2