A new stemofoline alkaloid, (2′S)-hydroxy-(11S,12R)-dihydrostemofoline (3), new stemofurans M-R (8-13), and known
compounds stemofoline (1), (2′S)-hydroxystemofoline (2), stemofuran E (4), stemofuran F (5), stemofuran J (6), and stilbostemin
F (7) have been isolated from the root extracts of Stemona aphylla. The structures and relative configurations of these new
compounds have been determined by spectroscopic data interpretation and from semisynthetic studies. These natural and
semisynthetic alkaloids were tested for acetylcholinesterase inhibitory activities and were found to be 10-20 times less active
than 1′,2′-didehydrostemofoline itself. Stemofurans 4, 6, 8, 11, and 13 were tested for their antibacterial and antifungal activities.
Three of these showed antibacterial activities against MRSA with MIC values of 15.6 μg/mL.