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PCR confirms multiple Wolbachia strain infection in Australian and international populations of the invasive stored-product psocid Liposcelis bostrychopila Badonnel

Journal Article


Abstract


  • Diagnostic Wolbachia polymerase chain reaction (PCR) primers were used to investigate whether Liposcelis bostrychophila individuals

    from Australian populations were infected by Wolbachia. Wolbachia PCR diagnostic primers were used to amplify Wolbachia from single

    individuals, individuals subjected to multiple displacement amplification (MDA), and from groups of about 50 pooled individuals each

    representing an Australian or United Kingdom (UK) L. bostrychophila population. Wolbachia PCR diagnostic primers successfully

    amplified DNA from pooled L. bostrychophila DNA extractions but not from individual L. bostrychophila DNA extractions or from

    MDA-subjected samples from both the Australian and UK populations. Use of restriction fragment-length polymorphism (RFLP)

    techniques produced cleavage sites in Australian and UK L. bostrychophila populations that were diagnostic of Wolbachia strains A and

    B. This study provides the first evidence of multiple Wolbachia infection with strains A and B in Australian L. bostrychophila

    populations.

Publication Date


  • 2007

Citation


  • Mikac, K. Maryann. (2007). PCR confirms multiple Wolbachia strain infection in Australian and international populations of the invasive stored-product psocid Liposcelis bostrychopila Badonnel. Journal of Stored Products Research, 43 (4), 594-597.

Scopus Eid


  • 2-s2.0-34547841393

Number Of Pages


  • 3

Start Page


  • 594

End Page


  • 597

Volume


  • 43

Issue


  • 4

Place Of Publication


  • http://www.elsevier.com/wps/find/journaldescription.cws_home/306/description#description

Abstract


  • Diagnostic Wolbachia polymerase chain reaction (PCR) primers were used to investigate whether Liposcelis bostrychophila individuals

    from Australian populations were infected by Wolbachia. Wolbachia PCR diagnostic primers were used to amplify Wolbachia from single

    individuals, individuals subjected to multiple displacement amplification (MDA), and from groups of about 50 pooled individuals each

    representing an Australian or United Kingdom (UK) L. bostrychophila population. Wolbachia PCR diagnostic primers successfully

    amplified DNA from pooled L. bostrychophila DNA extractions but not from individual L. bostrychophila DNA extractions or from

    MDA-subjected samples from both the Australian and UK populations. Use of restriction fragment-length polymorphism (RFLP)

    techniques produced cleavage sites in Australian and UK L. bostrychophila populations that were diagnostic of Wolbachia strains A and

    B. This study provides the first evidence of multiple Wolbachia infection with strains A and B in Australian L. bostrychophila

    populations.

Publication Date


  • 2007

Citation


  • Mikac, K. Maryann. (2007). PCR confirms multiple Wolbachia strain infection in Australian and international populations of the invasive stored-product psocid Liposcelis bostrychopila Badonnel. Journal of Stored Products Research, 43 (4), 594-597.

Scopus Eid


  • 2-s2.0-34547841393

Number Of Pages


  • 3

Start Page


  • 594

End Page


  • 597

Volume


  • 43

Issue


  • 4

Place Of Publication


  • http://www.elsevier.com/wps/find/journaldescription.cws_home/306/description#description