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Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin.

Journal Article


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Abstract


  • Class 1 integrons play a role in the emergence of multi-resistant bacteria by facilitating the recruitment of gene cassettes encoding antibiotic resistance genes. 512 E. coli strains sourced from humans (n = 202), animals (n = 304) and the environment (n = 6) were screened for the presence of the intI1 gene. In 31/79 integron positive E. coli strains, the gene cassette regions could not be PCR amplified using standard primers. DNA sequence analysis of 6 serologically diverse strains revealed atypical integrons harboured the dfrA5 cassette gene and only 24 bp of the integron 3'-conserved segment (CS) remained, due to the insertion of IS26. PCR targeting intI1 and IS26 followed by restriction fragment length polymorphism (RFLP) analysis identified the integron-dfrA5-IS26 element in 27 E. coli strains of bovine origin and 4 strains of human origin. Southern hybridization and transformation studies revealed the integron-dfrA5-IS26 gene arrangement was either chromosomally located or plasmid borne. Plasmid location in 4/9 E. coli strains and PCR linkage of Tn21 transposition genes with the intI1 gene in 20/31 strains, suggests this element is readily disseminated by horizontal transfer.

UOW Authors


  •   Hornitzky, Michael A (external author)
  •   Bettelheim, Karl A (external author)
  •   Kuzevski, Alexander (external author)
  •   Djordjevic, Steven P. (external author)
  •   Walker, Mark J. (external author)
  •   Dawes, Fay E.

Publication Date


  • 2010

Citation


  • Dawes, F. E., Kuzevski, A., Bettelheim, K., Hornitzky, M., Djordjevic, S. & Walker, M. J. (2010). Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin.. PloS one, 5 (9), 1-6.

Scopus Eid


  • 2-s2.0-77958608893

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1734&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/695

Has Global Citation Frequency


Number Of Pages


  • 5

Start Page


  • 1

End Page


  • 6

Volume


  • 5

Issue


  • 9

Abstract


  • Class 1 integrons play a role in the emergence of multi-resistant bacteria by facilitating the recruitment of gene cassettes encoding antibiotic resistance genes. 512 E. coli strains sourced from humans (n = 202), animals (n = 304) and the environment (n = 6) were screened for the presence of the intI1 gene. In 31/79 integron positive E. coli strains, the gene cassette regions could not be PCR amplified using standard primers. DNA sequence analysis of 6 serologically diverse strains revealed atypical integrons harboured the dfrA5 cassette gene and only 24 bp of the integron 3'-conserved segment (CS) remained, due to the insertion of IS26. PCR targeting intI1 and IS26 followed by restriction fragment length polymorphism (RFLP) analysis identified the integron-dfrA5-IS26 element in 27 E. coli strains of bovine origin and 4 strains of human origin. Southern hybridization and transformation studies revealed the integron-dfrA5-IS26 gene arrangement was either chromosomally located or plasmid borne. Plasmid location in 4/9 E. coli strains and PCR linkage of Tn21 transposition genes with the intI1 gene in 20/31 strains, suggests this element is readily disseminated by horizontal transfer.

UOW Authors


  •   Hornitzky, Michael A (external author)
  •   Bettelheim, Karl A (external author)
  •   Kuzevski, Alexander (external author)
  •   Djordjevic, Steven P. (external author)
  •   Walker, Mark J. (external author)
  •   Dawes, Fay E.

Publication Date


  • 2010

Citation


  • Dawes, F. E., Kuzevski, A., Bettelheim, K., Hornitzky, M., Djordjevic, S. & Walker, M. J. (2010). Distribution of class 1 integrons with IS26-mediated deletions in their 3'-conserved segments in Escherichia coli of human and animal origin.. PloS one, 5 (9), 1-6.

Scopus Eid


  • 2-s2.0-77958608893

Ro Full-text Url


  • http://ro.uow.edu.au/cgi/viewcontent.cgi?article=1734&context=scipapers

Ro Metadata Url


  • http://ro.uow.edu.au/scipapers/695

Has Global Citation Frequency


Number Of Pages


  • 5

Start Page


  • 1

End Page


  • 6

Volume


  • 5

Issue


  • 9